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目的观察螺旋藻多糖对糖尿病大鼠的降糖作用,并从氧化性损伤角度探讨其作用机制。方法雄性SD大鼠腹腔注射链尿佐菌素制备糖尿病模型,将造模成功大鼠按照血糖值和体重随机分为糖尿病模型组、阳性对照组(格列本脲,20 mg/kg)、螺旋藻多糖高剂量组(200 mg/kg)和低剂量组(100 mg/kg),经口灌胃给药,另设正常对照组,时间为8周,观察螺旋藻多糖对糖尿病大鼠体重、血糖、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的影响。结果模型组大鼠体重和血糖分别为(219.0±18.6)g,(27.6±2.4)mmol/L,血清SOD活力和MDA含量分别为(130.88±17.82)U/ml)、(11.24±0.93)nmol/ml;螺旋藻多糖高、低剂量组的体重和血糖分别为(273.0±26.9)g、(9.2±4.4)mmol/L和(270.1±43.4)g、(15.4±3.7)mmol/L,与模型组相比各剂量组大鼠体重明显增加(P<0.01),血糖显著下降(P<0.01),螺旋藻多糖高、低剂量组的SOD活力和MDA含量分别为(189.52±11.36)U/ml(5.87±0.61)nmol/ml和(155.72±19.0)U/ml、(6.65±1.02)nmol/ml,与模型组相比各剂量组血清中SOD活力均有提高(P<0.05),MDA含量显著下降(P<0.01)。结论螺旋藻多糖具有降血糖的作用,从氧化性损伤的角度分析,可认为其降糖的作用机制是通过增强SOD活力和降低MDA含量实现的。
Objective To observe the hypoglycemic effect of Spirulina platensis polysaccharide on diabetic rats and to explore its mechanism from oxidative damage. Methods Male Sprague-Dawley rats were injected intraperitoneally with streptozotocin to prepare a diabetic model. The successful model rats were randomly divided into diabetic model group, positive control group (glibenclamide, 20 mg / kg), spiral Algae polysaccharide high dose group (200 mg / kg) and low dose group (100 mg / kg), oral gavage, another set of normal control group, the time was 8 weeks, observed spirulina polysaccharide on diabetic rats body weight, Blood glucose, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Results The body weight and blood glucose in the model group were (219.0 ± 18.6) g and (27.6 ± 2.4) mmol / L respectively, and the serum SOD activity and MDA content were (130.88 ± 17.82) U / ml and 11.24 ± 0.93 nmol / ml. The body weight and blood glucose of high and low dose spirulina polysaccharide groups were (273.0 ± 26.9) g, (9.2 ± 4.4) mmol / L and (270.1 ± 43.4) g and (15.4 ± 3.7) mmol / L, Compared with the model group, body weight of rats in each dose group increased significantly (P <0.01), blood glucose decreased significantly (P <0.01), SOD activity and MDA content of high and low dose Spirulina platensis polysaccharide groups were (189.52 ± 11.36) U / (5.87 ± 0.61) nmol / ml and (155.72 ± 19.0) U / ml and (6.65 ± 1.02) nmol / ml, respectively. Compared with the model group, the activity of SOD in each dose group increased (P <0.05) Content decreased significantly (P <0.01). Conclusion Spirulina platensis polysaccharide has a hypoglycemic effect, oxidative damage from the point of view, can be considered hypoglycemic mechanism by enhancing SOD activity and reduce the content of MDA to achieve.