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探讨尿激酶型纤溶酶原激活物受体(UPAR)mRNA、血管内皮细胞生长因子(VEGF)和癌基因c-erbB2在肝细胞癌(HCC)的表达与其病理学特性和肿瘤转移的关系。采用原位杂交方法检测肝细胞癌UPA mRNA表达,免疫组化法检测VEGF和c-erbB2的表达。肝癌转移组的UPAR mRNA、VEGF和c-erbB2阳性表达率及表达强度均明显高于无转移组,二者相比均有显著性差异(P<0.05),但它们的表达与肝癌的大小、分化程度却无关;UPAR mRNA与VEGF及c-erbB2的表达强度均呈正相关(r=0.656、0.653,P<0.05)。UPA mRNA、VEGF和c-erbB2的表达可作为肝细胞癌的侵袭表型、判断疗效以及估计预后的重要指标。
To investigate the relationship between the expression of urokinase-type plasminogen activator receptor (UPAR) mRNA, vascular endothelial growth factor (VEGF) and oncogene c-erbB2 in hepatocellular carcinoma (HCC) and their pathological features and tumor metastasis. The expression of UPA mRNA in hepatocellular carcinoma was detected by in situ hybridization and the expressions of VEGF and c-erbB2 were detected by immunohistochemistry. The positive expression rates of UPAR mRNA, VEGF and c-erbB2 in hepatocellular carcinoma group were significantly higher than those in non-metastasis group (P <0.05), but their expressions were correlated with the size, UPAR mRNA was positively correlated with the expression of VEGF and c-erbB2 (r = 0.656,0.653, P <0.05). The expression of UPA mRNA, VEGF and c-erbB2 can be used as the invasive phenotype of hepatocellular carcinoma, to determine the efficacy and prognosis of an important indicator.