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目的 了解膀胱移行细胞癌中细胞凋亡与细胞增殖的关系 ,以及与相关基因蛋白表达的关系及其预后意义。方法 利用TUNEL法及免疫组化S P法检测 30例膀胱移行细胞癌及 10例“正常膀胱粘膜”中的细胞凋亡指数(AI)以及Bcl 2、p5 3的表达 ,同时计算核分裂指数 (MI)。结果 AI与MI呈显著的正相关 (r =0 .90 7,P <0 .0 1) ;AI、MI和Bcl 2、p5 3蛋白表达和组织学分级有关 ;AI、Bcl 2、p5 3的表达与 5年生存率有关。结论 膀胱癌中细胞凋亡与细胞增殖有关 ,AI、Bcl 2、p5 3高表达者预后及疗效较差
Objective To investigate the relationship between apoptosis and cell proliferation in transitional cell carcinoma of the bladder and its relationship with the expression of related genes and their prognostic significance. Methods TUNEL and immunohistochemical SP methods were used to detect the apoptosis index (AI) and the expression of Bcl 2, p 5 3 in 30 cases of transitional cell carcinoma of the bladder and 10 cases of normal bladder mucosa. The mitotic index (MI) . Results There was a significant positive correlation between AI and MI (r = 0.907, P <0.01). The expressions of AI, MI, Bcl 2 and p 5 3 were correlated with histological grade. The expressions of AI, Bcl 2 and p 5 3 Expression and 5-year survival rate. Conclusions Apoptosis in bladder cancer is related to cell proliferation. The prognosis and the curative effect of AI, Bcl 2, p 5 3 overexpression are poor