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对二系杂交水稻亲本籼型恢复系 2 88,198,112 5和籼型不育系株 1- s进行了离体培养和遗传转化的研究 ,建立了这些籼稻新品系的胚性愈伤组织高频诱导与再生系统及其基因枪转化系统 .它们的胚性愈伤组织诱导率分别可达 87.4 % ,90 .6% ,78.9%和 88.0 % ;其分化率分别可达 60 .0 % ,4 5 .8% ,4 6.9%和 60 .6% ;其基因枪转化率分别可达 16.69% ,2 .65 % ,2 .0 4 %和 10 .78% ,并获得了一批可育的水稻转基因植株
The in vitro culture and genetic transformation of indica restorer lines 888,198,1125 and indica sterile line 1s of two-line hybrid rice were studied in vitro and the induction of embryogenic callus induction Regeneration system and its gene gun transformation system.The embryogenic callus induction rates were up to 87.4%, 90.6%, 78.9% and 88.0% respectively, and their differentiation rates were 60.0% and 45.8% %, 4 6.9% and 60.6%, respectively. The gene gun conversion rates were 16.69%, 2.65%, 2.04% and 10.78% respectively, and a number of fertile rice transgenic plants were obtained