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目的：探讨细胞凋亡及增殖细胞核抗原（ＰＣＮＡ）与子宫颈癌的关系。方法：采用原位末端标记法（ＴＵＮＥＬ）检测细胞凋亡，采用免疫组化技术检测ＰＣＮＡ和Ｐ５３蛋白。结果：３２例子宫颈鳞癌组织中，细胞凋亡、Ｐ５３蛋白和ＰＣＮＡ阳性率分别为６２．５０％，５３．１３％和６５．６３％。表达Ｐ５３蛋白的细胞与表达ＰＣＮＡ细胞分布区域基本一致，与细胞凋亡无明显相关性。正常子宫颈组织只有上皮组织内有少数凋亡细胞和表达ＰＣＮＡ的细胞，显微镜下每视野阳性细胞数均不超过１０％。结论：Ｐ５３蛋白可能通过促进细胞增殖，抑制细胞凋亡而参与子宫颈癌发生与发展。
Objective: To investigate the relationship between apoptosis and proliferating cell nuclear antigen (PCNA) and cervical cancer. Methods: Apoptosis was detected by TUNEL. PCNA and P53 protein were detected by immunohistochemistry. Results: The positive rates of apoptosis, P53 protein and PCNA in 32 cases of cervical squamous cell carcinoma were 62.50%, 53.13% and 65.63% respectively. P53 protein-expressing cells and the distribution of PCNA cells are basically the same, and no significant correlation with apoptosis. Normal cervical tissue only a small number of apoptotic cells and PCNA-expressing cells in epithelial tissue, the number of positive cells per field under the microscope, no more than 10%. Conclusion: P53 protein may participate in the development and progression of cervical cancer by promoting cell proliferation and inhibiting apoptosis.