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目的合成姜黄素的硒化衍生物并研究其体外抗肿瘤活性。方法用姜黄素和氯氧化硒反应合成目标化合物,通过氢化物发生-石墨炉原子吸收光谱测定了合成物中的硒含量,用UV、IR、MS、1H-NMR和13C-NMR表征其结构;用姜黄素和顺铂做对照,采用四氮唑盐(MTT)法考察目标化合物对胃癌(human gastric cancer BGC823cells,BGC823)细胞、鼻咽癌(human nasopharyngeal carcinoma CNE cells,CNE)、白血病(human leukemic HL-60cells,HL-60)、口腔上皮癌(human oral epithelial carcinoma KB cells,KB)、结肠癌(human colon carcinoma LS174T cells,LS174T)、前列腺癌(human prostate cancer PC3cells,PC3)及宫颈癌(human cervical cancer cell line HELA cells,HELA)的细胞毒活性。结果确认目标化合物为一种新化合物二姜黄素硒氧配合物;对上述细胞株的IC50分别为45.71,>100,30.09,3.18,>100,21.76,2.54mg·L-1,对KB和HELA均表现出比姜黄素强的抑制活性,对HELA的抑制活性比顺铂要高。结论合成出一个新化合物二姜黄素硒氧配合物;该化合物对部分肿瘤细胞有很强的抑制活性;有关其硒化衍生物对肿瘤细胞生长的抑制机制有待进一步研究。
Objective To synthesize the selenium derivatives of curcumin and study its antitumor activity in vitro. Methods The target compounds were synthesized by the reaction of curcumin and chlorine oxychloride. The content of selenium was determined by hydride generation - graphite furnace atomic absorption spectrometry. The structures of the compounds were characterized by UV, IR, MS, 1H-NMR and 13C-NMR. Using curcumin and cisplatin as control, the effect of the target compound on human gastric cancer BGC823cells (BGC823) cells, human nasopharyngeal carcinoma (CNE), leukemia HL-60cells, HL-60), human oral epithelial carcinoma KB cells (KB), human colon carcinoma LS174T cells (LS174T), prostate cancer PC3cells (PC3) cervical cancer cell line HELA cells, HELA). The results confirmed that the target compound was a new compound curcumin selenium oxygen complex; IC50 of the above cell lines were 45.71,> 100,30.09,3.18,> 100,21.76,2.54mg · L-1, KB and HELA All showed a stronger inhibitory activity than curcumin and a higher inhibitory activity against HELA than cisplatin. Conclusion A novel compound, curcumin selenium oxygen complex, was synthesized. The compound has strong inhibitory activity on some tumor cells. The mechanism of its inhibitory effect on the growth of tumor cells remains to be further studied.