论文部分内容阅读
目的:研究藤黄对人结肠癌细胞的诱导凋亡作用及相关分子机制。方法:采用Annexin V-FITC/PI双染法、流式细胞法、免疫细胞化学染色法检测藤黄干预后对结肠癌细胞株HCT116和SW480的细胞凋亡、细胞周期及凋亡相关蛋白BAX、Bcl-2以及p53的影响。结果:1.25μg/ml、2.5μg/ml藤黄干预HCT116细胞48h,细胞凋亡率为11.28%、27.31%,1.25μg/ml、2.5μg/ml及5μg/ml藤黄干预SW480细胞48h,细胞凋亡率分别为15.84%、20.71%、35.61%,与对照组比较差异均有统计学意义(P<0.05)。0.625μg/ml、1.25μg/ml、2.5μg/ml藤黄干预HCT116细胞48h,2.5μg/ml、5μg/ml藤黄干预SW480细胞48h,G2/M期细胞所占比例与对照组比较差异有统计学意义;当干预浓度为2.5μg/ml时,HCT116细胞S期细胞所占比例与对照组比较差异有统计学意义。藤黄干预后HCT116和SW480细胞的BAX和p53蛋白表达明显上调,Bcl-2蛋白表达明显下调。结论:藤黄对人结肠癌细胞HCT116和SW480有明显的促凋亡作用,呈浓度依赖性,其机制可能和调节BAX、Bcl-2与p53蛋白的表达,产生G2/M期及S期阻滞有关。
Objective: To study the apoptosis-inducing effect of Garcinia cambogia on human colon cancer cells and its related molecular mechanisms. Methods: The apoptosis, cell cycle and the expression of apoptosis-related proteins BAX, HCT116 and SW480 in HCT116 and SW480 cells were detected by Annexin V-FITC / PI double staining, flow cytometry and immunocytochemical staining Bcl-2 and p53. HCT116 cells were treated with 1.25μg / ml, 2.5μg / ml Garcinia cambogia for 48h, the apoptotic rates were 11.28%, 27.31%, 1.25μg / ml, 2.5μg / ml and 5μg / The apoptotic rates were 15.84%, 20.71% and 35.61%, respectively, which were significantly different from the control group (P <0.05). HCT116 cells treated with 0.625μg / ml, 1.25μg / ml, 2.5μg / ml Garcinia chinensis for 48h, 2.5μg / ml, 5μg / ml Garcinia chinensis intervention SW480 cells 48h, the proportion of G2 / M phase cells compared with the control group Statistical significance; when the intervention concentration of 2.5μg / ml, HCT116 cells in S phase cells compared with the control group, the difference was statistically significant. The expression of BAX and p53 in HCT116 and SW480 cells was significantly up-regulated and the expression of Bcl-2 protein was significantly down-regulated after the intervention of Garcinia cambogia. Conclusion: Garcinia cambogia has obvious apoptosis-inducing effects on HCT116 and SW480 cells in a concentration-dependent manner. Its mechanism may regulate the expressions of BAX, Bcl-2 and p53 proteins and induce G2 / M phase and S phase obstruction Hysteresis related.