Elucidation of O-Phosphoryl and N-Phosphoryl Amino Acids by Electrospray Ionization Tandem Mass Spec

• 作 者: 张建臣 曹书霞 徐军 廖新成 赵玉芬
• 机 构: The Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University Zhengzhou, Hen
• 出 处: Chinese Journal of Chemistry
• 发表日期: 2004年08期
• 关键词: fragmentation phospho dissociation propylene hydroxyl distinguished threonine re

Mass spectroscopic characteristics of phosphoryl amino acids were studied in detail by positive and negative electrospray ionization mass spectrometry (ESI-MS) in conjunction with tandem mass spectrometry (MS/MS). Be- sides N-diisopropyloxyphosphoryl amino acids (N-DIPP-AA), O-phospho- and O-diisopropyloxyphosphoryl amino acids (O-DIPP-AA) were studied and compared to N-DIPP-AA. The fragmentation pathways of [M+H]+, [M+Na]+ and [M-H]- ions of phosphoryl amino acids were summarized. In addition to several similar patterns, each of them showed its characteristic fragmention.

Mass spectroscopic characteristics of phosphoryl amino acids were studied in detail by positive and negative electrospray ionization mass spectrometry (ESI-MS) in conjunction with tandem mass spectrometry (MS / MS). Be- sides N- diisopropyloxyphosphoryl amino acids (N-DIPP- AA ), O-phospho- and O-diisopropyloxyphosphoryl amino acids (O-DIPP-AA) were studied and compared to N-DIPP-AA. The fragmentation pathways of [M + H] +, [M + Na] + and [MH ] - ions of phosphoryl amino acids were summarized. In addition to several similar patterns, each of them showed its characteristic fragmention.