论文部分内容阅读
目的探讨核因子κBp65(NF-κBp65)蛋白及中性粒细胞活化肽-78(ENA-78)在子宫内膜异位症(内异症)发病中的作用。方法选取2008年10月至2009年2月在山西医科大学第二医院手术切除的卵巢子宫内膜异位囊肿25例,留取异位内膜与在位内膜组织,分为异位内膜组和在位内膜组,选取同期正常的子宫内膜22例为对照组。不同浓度的白细胞介素1β(IL-1β)及IL-1β+二硫氨基甲酸肽吡咯烷(PDTC)分别干预体外传代培养至第3代的在位、异位及正常内膜基质细胞。免疫细胞化学方法及酶联免疫吸附实验(ELISA)检测3组子宫内膜基质细胞NF-κBp65蛋白活化程度及其培养上清液中ENA-78浓度。用IL-1β、IL-1β+PDTC检验各组内膜基质细胞分泌ENA-78及NF-κBp65蛋白的活化程度。结果在位及异位子宫内膜基质细胞培养上清液中ENA-78浓度明显高于正常子宫内膜基质细胞,差异有统计学意义(P<0.01);在位及异位子宫内膜基质细胞之间比较,异位子宫内膜基质细胞培养上清液中的ENA-78浓度高于在位子宫内膜基质细胞,差异有统计学意义(P<0.01),分别给予IL-1β、IL-1β+PDTC干预后,正常子宫内膜基质细胞上清液中的ENA-78浓度无明显变化(P>0.05);而在位及异位内膜基质细胞的上清液中,ENA-78浓度在IL-1β干预后最高,明显高于未干预时及IL-1β+PDTC干预后,差异有统计学意义(P<0.01)。3组中,在位及异位内膜基质细胞的NF-κBp65蛋白的活化程度显著高于对照组,差异有统计学意义(P<0.05);在位及异位子宫内膜基质细胞NF-κBp65蛋白的活化程度IL-1β干预后最高,显著高于未干预时及IL-1β+PDTC干预后,差异有统计学意义(P<0.05)。结论 IL-1β可诱导在位及异位子宫内膜基质细胞NF-κBp65活化,使ENA-78的表达增高。PDTC可抑制此作用,说明NF-κBp65是关键性的调控因子,可能在内异症的发病过程中起到“闸门”的作用,对ENA-78以及其他细胞因子起到重要的调控作用。
Objective To investigate the role of nuclear factor κBp65 (NF-κBp65) and neutrophil activating peptide-78 (ENA-78) in the pathogenesis of endometriosis (endometriosis). Methods Twenty-five patients with ovarian endometriosis surgically resected from the Second Hospital of Shanxi Medical University from October 2008 to February 2009 were selected, and the ectopic endometrium and eutopic endometrium were collected for ectopic endometrium Group and eutopic endometrium group, select the same period of normal endometrium in 22 cases as control group. Interleukin-1β (IL-1β) and IL-1β + dithiocarbamate pyrrolidine (PDTC) were separately in vitro cultured to the third generation of eutopic and ectopic endometrial stromal cells in vitro. Immunocytochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the activation of NF-κB p65 protein in the endometrial stromal cells and the concentration of ENA-78 in the culture supernatant. The levels of ENA-78 and NF-κB p65 protein secreted by endometrial stromal cells in each group were examined by IL-1β, IL-1β + PDTC. Results The concentration of ENA-78 in eutopic and ectopic endometrial stromal cell culture supernatants was significantly higher than that of normal endometrial stromal cells (P <0.01). Eutopic and ectopic endometrial stromal cells Compared with eutopic endometrial stromal cells, the concentration of ENA-78 in ectopic endometrial stromal cell culture supernatant was significantly higher than that in eutopic endometrial stromal cells (P <0.01), and IL-1β, IL The level of ENA-78 in the normal endometrial stromal cell supernatant did not change significantly after intervention with -1β + PDTC (P> 0.05). However, in the eutopic and ectopic stromal cells, ENA-78 The concentration of IL-1β was the highest after intervention, which was significantly higher than that of non-intervention and IL-1β + PDTC (P <0.01). In group 3, the activation of NF-κBp65 protein in eutopic and ectopic stromal cells was significantly higher than that in control group (P <0.05); in eutopic and ectopic endometrial stromal cells, NF- The activation level of κBp65 protein was the highest after intervention of IL-1β, which was significantly higher than that of non-intervention and IL-1β + PDTC (P <0.05). Conclusion IL-1β can induce the activation of NF-κBp65 in eutopic and ectopic endometrial stromal cells and increase the expression of ENA-78. PDTC can inhibit this effect, indicating that NF-κBp65 is a key regulator, may play a “gate” in the pathogenesis of endometriosis play an important regulatory role of ENA-78 and other cytokines .