论文部分内容阅读
目的:测定复方克比热提片中没食子酸的含量。方法:采用高效液相色谱法,ECOSIL C18色谱柱(250×4.60mm,5μm),柱温30℃,流动相为甲醇-0.2%HPO3(5:95)为流动相,检测波长为273nm,流速为1.0m L/min。结果:没食子酸在10.94~54.70μg/m L之间呈良好的线性关系,r=0.9997,平均回收率为101.63%,RSD为0.26%。结论:该方法具有操作简单,灵敏,准确的优点,分离效果显著,可用于余甘子的含量测定和本产品质量控制。
OBJECTIVE: To determine the content of gallic acid in Compound Kobuca tablets. Methods: High performance liquid chromatography with ECOSIL C18 column (250 × 4.60mm, 5μm) was used with a mobile phase of methanol-0.2% HPO3 (5:95) as mobile phase at a column temperature of 30 ℃. The detection wavelength was 273nm and the flow rate 1.0 m L / min. Results: There was a good linear relationship between gallic acid and 10.94 ~ 54.70μg / m L, r = 0.9997, the average recovery was 101.63% and the RSD was 0.26%. Conclusion: The method has the advantages of simple operation, sensitive and accurate. The separation effect is significant. It can be used for the determination of Phyllanthus emblica and the quality control of this product.