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To detect the pathogenesis of age-related cataract, we analyzed the expression of TGF-β_2mRNA, proliferation cell nuclear antigen (PCNA), Bcl-2/Bax, fibronection (FN), vimentin protein and the density of lens epithelial cells(LECs) of nuclear cataract, cortex cataract and normal LECs. Results showed that the expression level of TGF-β_2mRNA, FN and vimentin protein was higher in LECs of cortex cataract than that of nuclear cataract and normal lens. But the level of Bcl-2/Bax and PCNA was on the contrary. The density of LECs was (4250.63±275.05)/mm~2 in cortex cataract. It was (5438.40± 262.30)/mm~2 in nuclear cataract, and (5368.63 ±211.07)/mm~2 in normal LECs resepectively. There was significant difference between cortex and nuclear cataract (p<0.05 ). These suggested that TGF-β_2 might take an important part in the process of age-related cataract. Age-related nuclear cataract was related to the proliferation LECs. While cortex cataract was related to the apoptosis and transdifferentiation of LECs.
To detect the pathogenesis of age-related cataract, we analyzed the expression of TGF-β 2 mRNA, proliferation cell nuclear antigen (PCNA), Bcl-2 / Bax, fibronection (FN), vimentin protein and the density of lens epithelial cells (LECs) of nuclear cataract, cortex cataract and normal LECs. Results showed that the expression level of TGF-β_2 mRNA, FN and vimentin protein was higher in LECs of cortex cataract than that of nuclear cataract and normal lens. But the level of Bcl-2 / Bax The density of LECs was (4250.63 ± 275.05) / mm 2 in cortex cataract. It was (5438.40 ± 262.30) / mm ~ 2 in nuclear cataract, and (5368.63 ± 211.07) / mm 2 There was a significant difference between cortex and nuclear cataract (p <0.05). These suggested that TGF-β_2 might take an important part in the process of age-related cataract. Age-related nuclear cataract was related to the proliferation LECs. While cortex cataract was related to the apoptosis and transdiffe rentiation of LECs.