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目的 探讨干细胞白血病 (SCL)基因在急性淋巴细胞白血病 (AL L)中的表达情况及临床意义。 方法 采用 RT- PCR技术检测 SCL 及其融合基因 SIL- SCL 在 AL L 细胞系和患者的表达情况 ,并观察其临床预后关系。 结果 (1) SCL 基因在 AL L 的表达率为 5 1.2 % ,SIL- SCL 基因仅在 T- AL L 中表达 ,占 10 .5 %。(2 ) SCL 阳性表达的 AL L 患者完全缓解率和生存率减低。 结论 (1) SCL 基因对 AL L 的发生有一定作用 ,其阳性表达是 AL L预后不良因素之一。 (2 ) SCL 异常激活的方式以过量表达常见 ,基因重排较少见 ,染色体易位罕见
Objective To investigate the expression and clinical significance of stem cell leukemia (SCL) gene in acute lymphoblastic leukemia (AL L). Methods RT-PCR was used to detect the expression of SCL and its fusion gene SIL-SCL in AL cell lines and patients, and to observe its clinical prognosis. Results (1) The expression rate of SCL gene in AL L was 5.2%, while that in SIL-SCL gene was only expressed in T-AL L, accounting for 10.5%. (2) The complete remission rate and survival rate of ALL patients with SCL positive expression decreased. Conclusion (1) The SCL gene has a certain effect on the occurrence of AL and its positive expression is one of the bad prognostic factors of AL L. (2) The way of SCL abnormal activation is common in overexpression, rare gene rearrangement, rare chromosomal translocation