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目的探讨汽油中挥发性有机物(volatile organic matters,VOCs)对肺组织的损伤作用。方法将SPF级雄性昆明小鼠40只随机分为对照组和汽油低、中、高剂量组,每组10只。分别称取汽油1、2、4 g置于静式染毒柜中作为染毒的低、中、高剂量,待挥发30 min后将小鼠置于染毒柜中,每天染毒2 h,持续28 d。减重法测得低、中、高剂量组染毒柜内的VOCs浓度分别为9.524、18.873、34.746 mg/m~3。用试剂盒检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,并观察肺组织形态学变化。结果汽油高剂量组小鼠的肺组织MDA含量高于对照组,SOD和GSH-Px活力低于对照组,差异均有统计学意义(P<0.05)。各染毒组小鼠肺组织均可见肺泡壁增生变厚、炎细胞浸润。结论汽油VOCs可引起小鼠肺组织氧化损伤和病理学改变。
Objective To investigate the effect of volatile organic matters (VOCs) on lung tissue injury in gasoline. Methods Forty SPF male Kunming mice were randomly divided into control group and gasoline low, medium and high dose group, 10 rats in each group. Were weighed 1,2,4 g of gasoline were placed in the static exposure cabinet as a low, medium and high doses of exposure to be 30 minutes after the volatilization of the mice were exposed to the cabinet, the daily exposure to 2 h, sustained 28 d. The concentration of VOCs in the low, medium and high dose groups was 9.524, 18.873 and 34.746 mg / m ~ 3, respectively. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) were detected by kit, and the morphological changes of lung were observed. Results The content of MDA in lung tissue of high-dose gasoline group was higher than that of control group, while the activity of SOD and GSH-Px was lower than that of control group (P <0.05). The lung tissue of all exposure groups showed alveolar wall hyperplasia and thickening, inflammatory cell infiltration. Conclusion Gasoline VOCs can induce oxidative damage and pathological changes in lung tissue of mice.