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应用聚合酶链反应（ＰＣＲ）方法对４株肾综合征出血热（ＨＦＲＳ）病毒进行了分型。ＨＦＲＳ病毒的ＲＮＡ经逆转录为ＣＤＮＡ后用ＮＴＮ（野鼠型）、ＳＥＯ（家鼠型）两种引物进行体外扩增，经琼脂糖凝胶电泳，ＰＣＲ产物的ＮＤＡ片段与Ｍａｒｋ对照，证实扩增出的产物为ＨＦＲＳ病毒ＤＮＡ片段。ＰＣＲ分型与物异性单克隆抗体（ＭｃＡｂ）间接免疫荧光法分型作比较，结果相符合。
Four strains of hemorrhagic fever with renal syndrome (HFRS) virus were genotyped by polymerase chain reaction (PCR). The RNA of HFRS virus was amplified in vitro by reverse transcription of CDNA and NTN (wild type) and SEO (mouse type). The agarose gel electrophoresis and NDA fragment of PCR product were compared with Mark, confirming that amplification The product is an HFRS virus DNA fragment. PCR typing and heterosexual monoclonal antibody (McAb) indirect immunofluorescence typing for comparison, the results are consistent.