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本研究致力于筛选出小豆苗期抗旱性的生理指标并据此鉴定品种抗旱性。以白红3号、白红4号、白红6号、冀红352等小豆品种为材料,对各品种苗期进行不同浓度(5%、10%、20%)PEG模拟水分胁迫处理,在处理后的3、6、9d取样(功能叶片),测定各抗旱生理指标。结果表明:5%PEG处理后6d的超氧化物歧化酶(SOD)活性、5%PEG处理后6d的脱落酸(ABA)含量、10%PEG处理后6d的可溶性糖含量、5%PEG处理后3d的相对电导率、5%PEG处理后6d的超氧阴离子(O_2~-·)产生速率,均可作为鉴定小豆苗期抗旱性生理指标。利用上述抗旱生理指标,初步确定白红6号和白红4号为较抗旱品种,白红3号和冀红352的抗旱性较弱。
This research is devoted to screening physiological indexes of drought resistance of adzuki bean and to identify its drought resistance. The seedlings of different varieties (5%, 10%, 20%) of PEG were used to simulate water stress treatment with adzuki bean varieties such as Baihong No.3, Baihong No.4, Baihong No.6 and Jihong No.352, 3,6,9 d after sampling (functional leaves), determination of physiological indicators of drought. The results showed that the activities of superoxide dismutase (SOD), the contents of ABA at 6 d after 5% PEG treatment, the soluble sugar content at 6 d after 10% PEG treatment and the content of soluble sugar at 6 d after 5% PEG treatment The relative conductivity of 3d, and the generation rate of superoxide anion (O_2 ~ - ·) 6d after 5% PEG treatment could be used as the physiological indexes to identify the drought resistance of adzuki bean. Based on the physiological indexes of drought resistance, Baihong 6 and Baihong 4 were preliminarily determined to be more drought resistant than Baihong 3 and Jihong 352.