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目的探讨建立脾虚肝旺型功能性消化不良(FD)幼鼠模型的方法。方法 20只SD幼鼠随机分为2组,正常组予正常饮食,模型组予不规则喂养加夹尾刺激法造模,逢双日正常进食,逢单日禁食不禁水,连续2周后,在继续上述喂养的同时,用长海绵钳夹幼鼠尾巴远端1/3处,以不破皮为度,令其暴怒,寻衅与其他幼鼠撕打,以激怒全笼幼鼠。每次刺激20 min,每隔3 h刺激1次,每日3次,连续刺激1周。观察幼鼠的一般情况及饮水量、食量、体质量变化,测定胃肠排空率,并与正常组作对照。结果模型组幼鼠摄食量、饮水量减少,体质量减轻,与正常组比较差异有统计学意义(P<0.01);胃肠排空率下降,与正常组比较差异有统计学意义(P<0.01)。结论不规则喂养加夹尾刺激法制造的脾虚肝旺型(FD)幼鼠模型,符合FD动物模型的生物学特征,可用于实验研究。
Objective To explore a method of establishing a rat model of functional deficiency of liver and spleen with liver dysfunction (FD). Methods Twenty young SD rats were randomly divided into two groups. The normal group was given normal diet. The model group was fed irregular feeding and tail-tailed stimulation. The rats were fed on normal days and fasting for two days. While continuing the above feeding, a long sponge was used to clamp the distal 1/3 of the baby’s tail, so as not to break the skin, causing it to be furious, provocative and tearing with other young rats to irritate the whole cage. Each stimulation 20 min, once every 3 h stimulation, 3 times a day, continuous stimulation for 1 week. Observed the general situation of young rats and water intake, food intake, body weight changes, determination of gastrointestinal emptying rate, and with the control group. Results Compared with the normal group, the food intake, water intake and body weight of the model group decreased significantly (P <0.01) and the rate of gastrointestinal emptying decreased, which was significantly different from the normal group (P < 0.01). Conclusion Irregular feeding and tail-tailed stimulation of spleen-deficiency liver model (FD) rat model, in line with the biological characteristics of FD animal model can be used for experimental study.