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血浆中儿茶酚胺类化合物（ＣＡｓ）与二苯基硼酸－２－氨基乙酯在碱性条件下生成配合物，经液液提取后，与１，２－二苯基乙二胺（ＤＰＥ）在适宜的条件下发生衍生化反应，生成相应的二苯基喹喔啉衍生物。这些衍生物在岛津Ｓｈｉｍ－ＰａｃｋＣＬＣ－ＯＤＳ柱上，用甲醇－０．５０ｍｏｌ－１Ｔｒｉｓ－ＨＣＩ缓冲液等度洗脱，１６ｍｉｎ能完全分离；在Ｗａ－ｔｅｒｓＮｏｖａ－ｐａｃｋＣ１８柱上，用甲醇０．０１ｍｏｌ．Ｌ－１Ｔｒｉｓ－ＨＣＩ缓冲液等度洗脱，１０ｍｉｎ能完全分离；荧光检测波长：ｅｘ３５０ｎｍ，ｅｍ４８０ｎｍ。去甲肾上腺素（ＮＥ）、肾上腺素（Ｅ）和多巴胺（ＤＡ）的检测限分别为１０ｆｍｏｌ．１００ｕｌ－１．１０ｆｍｏｌ．１００ｕｌ－１和１２．５ｆｏｍｏｌ．１００ｕｌ－１。本方法用于正常人及疾病患者血浆中ＣＡｓ的测定，均得到满意结果。
The complexes of catecholamines (CAs) and 2-aminoethyl diphenylboronate in alkaline solution were obtained by liquid-liquid extraction and then reacted with 1,2-diphenylethylenediamine (DPE) Under the conditions of derivatization reaction to generate the corresponding diphenylquinoxaline derivatives. These derivatives were isocratically eluted on a Shimadzu Shim-Pack CLC-ODS column with methanol-0.50 mol-1 Tris-HCI buffer and completely separated over 16 min; on a Wa-tersNova-pack C18 column, . L-1Tris-HCI buffer isocratic elution, 10min can be completely separated; fluorescence detection wavelength: ex350nm, em480nm. The limits of detection for norepinephrine (NE), epinephrine (E) and dopamine (DA) were 10 fmol, respectively. 100 ul-1.10 fmol. 100 ul-1 and 12.5 fomol. 100ul-1. The method is applied to the determination of plasma CAs in normal subjects and patients with satisfactory results.