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The root development of Actinidia chinensis plantlets was studied in exposure to environmental stress of mechanical vibration at respectively 1 Hz, 2 Hz, 3 Hz, 4 Hz and 5 Hz. The plantlets exposed to vibration stimuli at all those frequencies have a larger total number and a larger total length of roots and a smaller permeability of root plasma-membrane, compared with those cultivated in an environment without vibration stress. Vibration at respectively 1 Hz, 2 Hz, 3 Hz and 4 Hz enhances root activity and the 3 Hz vibration is the most favorable. There is an obvious negative correlation between root activity and permeability of root plasma-membrane. The effects may be explained by the likelihood that mechanical vibration at an appropriate frequency facilitates roots absorbing water and minerals which are indispensable to inducing and synthesizing in roots some active substances favorable to growth. Nevertheless, overstress damages the integrity of root plasm-membrane, increases the permeability, and results in the disability of protecting root cells.