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以哲罗鱼(Hucho taimen)肾细胞为材料,采用胸腔注射PHA和秋水仙素溶液,空气干燥法制备了染色体,姬姆萨染色和核型分析。结果表明,哲罗鱼的二倍体染色体数目为2n=84,核型公式为:2n=18m+16sm+34st+16t,NF=118。
Chromosomes, Giemsa staining and karyotype analysis were prepared by air-drying method using Hucho taimen kidney cells as materials, by intrathoracic injection of PHA and colchicine solution. The results showed that the number of diploid chromosomes was 2n = 84 and karyotype was 2n = 18m + 16sm + 34st + 16t and NF = 118.