论文部分内容阅读
当原虫血症为70～90%时,从约氏和夏氏疟原虫感染的Balb/c小鼠取压积血0.1ml与0.5ml pH7.4 PBS混合,再加0.3ml弗氏完全佐剂乳化后,分4次肌肉注射免疫家兔,每2周一次,末次注射后1周采血。每ml血清用5×10~8正常鼠红细胞及3mg冻干的正常鼠血浆吸收。感染约氏或夏
When parasitemia was 70-90%, 0.1 ml of Balb / c mice infected with Johne & lt; (R) & gt; and Plasmodium malaria were mixed with 0.5 ml of pH 7.4 PBS and 0.3 ml Freund’s complete adjuvant After emulsification, rabbits were immunized intramuscularly in 4 times every 2 weeks, and blood was collected one week after the last injection. Per ml serum with 5 × 10 ~ 8 normal rat red blood cells and 3mg lyophilized normal rat plasma absorption. Infected with Johne’s or summer