论文部分内容阅读
骨髓基质细胞确切的辐射敏感性是一个尚有争论的问题。为此,Zuckerman KS 等对照射0、9、15、20、25、50、100、200或500Gy 的长期培养小鼠骨髓细胞贴壁融合基质细胞层进行了研究。所有受照射的培养物造血均迅速停止。并且,观察十周未见恢复。辐射剂量在100Gy 以下时,基质细胞看不出明显形态学改变。但是随着剂量加大,则出现细胞浆肿胀、细胞溶解和基质细胞的明显减少。照射9Gy 后两天内,RNA和蛋白质总合成下降35～60%,下降的主要原因可能是由于贴壁层中造血细胞的丢失。辐射剂量达100Gy
The exact radiosensitivity of bone marrow stromal cells is a matter of debate. To this end, Zuckerman KS and other irradiated 0,9,15,20,25,50,100,200 or 500Gy long-term cultured mouse bone marrow adherent stromal cell layer was studied. Hematopoiesis was stopped rapidly in all irradiated cultures. Also, no recovery was observed for ten weeks. Radiation doses below 100Gy, stromal cells showed no obvious morphological changes. However, as the dose increased, cytoplasmic swelling, cellular lysis and marked reduction of stromal cells occurred. The total RNA and protein synthesis decreased by 35-60% within two days after irradiation with 9Gy, the main reason for the decrease may be due to the loss of hematopoietic cells in the adherent layer. Radiation dose up to 100Gy