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目的研究caspase-9、-3在小鼠皮肤切创愈合过程中的表达及其变化规律。方法在小鼠背部正中制作皮肤全层切创模型,应用免疫组化染色技术观察切创及切创周边区内caspase-9、-3的表达情况,以无切创的小鼠皮肤作为对照。结果对照组中caspase-9、-3表达于表皮层,毛囊及皮脂腺。伤后3h损伤区及损伤周边区中可见少量多核粒细胞表达caspase-9、-3,6~24h,部分浸润的多核粒细胞和单核细胞caspase-9、-3阳性,此后caspase-9、-3阳性细胞逐渐以单核细胞和成纤维细胞为主。伤后caspase-9、-3阳性细胞率逐渐升高并在3d达到高峰,此后逐渐下降,至14d最低。结论caspase-9、-3可能引发正常小鼠皮肤表皮、毛囊和皮脂腺细胞的凋亡并参与正常皮肤细胞的自我更新;在小鼠皮肤切创愈合过程中,caspase-9、-3在多核粒细胞、单核细胞及成纤维细胞中表达,其时序性变化规律可望用于皮肤切创损伤时间的判定。
Objective To study the expression of caspase-9 and -3 in the incised wound healing of mice and their changes. Methods The full-thickness skin incision model was made in the middle of the back of the mice. The expression of caspase-9 and -3 in the peri-incision and incisional margins was observed by immunohistochemical staining. The skin of mice without incision was used as the control. Results In the control group, caspase-9 and -3 were expressed in the epidermis, hair follicles and sebaceous glands. A small number of multinucleated granulocytes showed caspase-9, -3, 6 ~ 24h, partially infiltrating multinucleated granulocytes and monocytes caspase-9, -3 in injured area and injured peripheral area at 3h after injury, -3 positive cells gradually monocytes and fibroblasts. After injury, the positive rate of caspase-9, -3 cells gradually increased and peaked at 3d, then decreased gradually and reached the lowest level at 14d. Conclusion Caspase-9 and -3 may induce the apoptosis of normal skin, hair follicles and sebaceous gland cells, and participate in the self-renewal of normal skin cells. During the skin incised wound healing, the expression of caspase-9 and -3 in multi-nuclear Cells, monocytes and fibroblasts, the timing of the law is expected to be used for skin incision injury time to determine.