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变形生长因子-α(TGF-α)和表皮生长因子(EGF)在胎儿胎盘的发展和功能方面都有重要作用,但 TGF-α和 EGF 的体内来源尚未确定。本研究旨在探讨 TGF-α和 EGF 是否由胎盘细胞合成,在体内是否通过自体分泌机理影响胎盘细胞的功能。人胎盘组织在妊娠早期通过刮宫获得,妊娠中、晚期在分娩时获得,并尽可能选择胎儿胎盘组织。对样品首先进行总 RNA 测定,其次用色谱法从总 RNA 中分离多腺苷酸阳性 RNA,用与 EGF
Transforming growth factor-alpha (TGF-alpha) and epidermal growth factor (EGF) play an important role in fetal placental development and function, but the in vivo source of TGF-alpha and EGF has not been established. The purpose of this study was to investigate whether TGF-α and EGF are synthesized by placental cells and whether placental cell function is affected by autologous secretion in vivo. Human placenta tissue is obtained by curettage in early gestation, obtained during childbirth in the second and third trimester of pregnancy, and chooses fetal placenta tissue as much as possible. Total RNA was first assayed for the samples, followed by chromatographic separation of poly Adenylate positive RNA from total RNA,