Neutrophil engagement and septic challenge in acute experimental pancreatitis in rats

• 作 者: Stanislaw Ha(c) Marek Dobosz Jan J Kaczor Robert Rzepko Ewa Aleksandrowicz-Wrona Zdzis(l)aw Wajda Zbigniew (S)ledziński Jacek Krajewski
• 机 构: Department of General Endocrine and Transplant Surgery,Medical University of Gdańsk, Poland“,”Depart
• 出 处: 世界胃肠病学杂志（英文版）
• 发表日期: 2005年41期
• 关键词: Acute pancreatitis Polymorphonuclear cells Immunomodulation Septic shock

AIM: To investigate the influence of neutrophil adhesion molecule blockade with monoclonal antibody (MoAb CD11b) and E. coli lipopolysaccharide (LPS)administration on experimental acute pancreatitis (AP).METHODS: AP was induced by four ip injections of cerulein (Cn) at 1-h intervals. MoAb CD 11b and LPS were administered at the beginning of the experiment.RESULTS: The neutrophil count and chemiluminescence were diminished at the beginning of AP. The oxidative stress parameters were found within the pancreatic gland. MoAb CD 11b used for AP resulted in a significant reduction of pancreatic infiltration and pancreatitis oxidative stress parameters. Serum interleukin-6 (IL-6)was not detected in AP animals, whereas high serum IL-6 concentration was noted only in animals receiving LPS.CONCLUSION: Neutrophils are involved in pancreatic damage in the early stage of AP. Neutrophil infiltration reduction protects the pancreatic gland from destruction during AP. LPS does not change the early course of Cn pancreatitis in rats.