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共价耦联HCG抗体的醋酸纤维素膜,当表面发生抗体与抗原复合的免疲化学反应后、膜的电荷密度向负值减小的方向移动,膜电位随抗原浓度的增大,向正方向移动。根据膜电位与抗原浓度之间的关系,可构成测定HCG的膜电位免疫传感器。利用夹心原理,在膜表面形成抗体-抗原-酶标抗体的夹心结构所组成的酶免疫传感器,具有很高的检测灵敏度,且与放射免疫测定显著相关。
When cellulose acetate membrane covalently coupled with HCG antibody, the charge density of the membrane moved in the direction of decreasing negative value when there was no chelation reaction between antibody and antigen complexed on the surface. As the concentration of antigen increased, Move in the direction. According to the relationship between the membrane potential and the antigen concentration, a membrane potential immunosensor for measuring HCG can be constructed. The enzyme immunoassay, consisting of a sandwich structure of antibody-antigen-enzyme-labeled antibody on the membrane surface, has high detection sensitivity and is significantly related to radioimmunoassay.