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以大白菜成青２号为材料，从萌发５天的无菌苗下胚轴分离原生质体，然后以附加８．２％葡萄糖，０．４ｍｇ／Ｌ２，４－Ｄ，０．４ｍｇ／ＬＮＡＡ和０．５ｍｇ／Ｌ６－ＢＡ的改良ＫＭ８ｐ液体培养基进行浅层培养．培养１３天后，用含５．２％葡萄糖的ＫＭ８ｐ培养基以１∶１进行稀释．４～６周后，再生细胞分裂并形成愈伤组织，直径约０．４～０．５ｍｍ的愈伤组织经ＭＳ培养基（附加２％蔗糖，０．８％的琼脂，１．５ｍｇ／Ｌ６－ＢＡ，０．５ｍｇ／ＬＮＡＡ和０．２ｍｇ／Ｌ２，４－Ｄ）诱导植株分化．５９％的愈伤组织可分化形成芽，分化的芽经诱导生根发育成完整的植株．０．８ｍｇ／ＬＩＢＡ和４００ｍｇ／Ｌ的羧苄青霉素对分化再生有促进作用．
Using Chinese cabbage Chengqing 2 as material, protoplasts were isolated from the hypocotyls of sterile seedlings for 5 days and then cultured in the medium supplemented with 8.2% glucose, 0.4mg / L 2,4-D, 0.4mg / L NAA and 0.5mg / L 6-BA improved KM8p liquid medium for shallow culture. After 13 days of culture, the cells were diluted 1: 1 with KM8p medium containing 5.2% glucose. After 4 to 6 weeks, the regenerated cells split and formed callus. Callus with diameter of about 0.4-0.5 mm was cultured in MS medium supplemented with 2% sucrose, 0.8% agar, 1.5 mg / L 6 -BA, 0.5 mg / L NAA and 0.2 mg / L 2,4-D). 59% of the callus can differentiate to form buds, and differentiated buds induce rooting to complete plants. 0.8mg / LIBA and 400mg / L of carbenicillin on the promotion of differentiation and regeneration.