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首先制备表面带有氨基的聚氨酯基材,再采用层层自组装技术,将两种生物大分子修饰剂(羧甲基纤维素和溶菌酶)层层自组装进行聚氨酯材料的表面修饰。采用原子力显微镜、水接触角测量仪、抗菌活性测试和溶血试验等方法对层层自组装修饰前后的聚氨酯材料的表面性能和生物性能进行了分析。测试结果表明,经过羧甲基纤维素与溶菌酶层层自组装表面修饰后的聚氨酯材料的亲水性提高,未修饰聚氨酯的水接触角为73.0°,而具有5个双分子修饰层的聚氨酯膜片的水接触角为42.3°;与未修饰的聚氨酯相比,层层自组装修饰后的聚氨酯对金黄色葡萄球菌的抗菌活性提高了41.4%;此外,经过层层自组装表面修饰的聚氨酯材料具有更好的血液相容性。
Firstly, a polyurethane substrate with amino groups on the surface is prepared, and two layers of biological macromolecule modifiers (carboxymethylcellulose and lysozyme) are self-assembled to modify the surface of the polyurethane by layer-by-layer self-assembly technology. The surface properties and biological properties of the polyurethane materials before and after self-assembly modification were analyzed by atomic force microscopy, water contact angle measurement, antibacterial activity test and hemolysis test. The test results show that the hydrophilicity of the polyurethane material modified by self-assembled carboxymethyl cellulose and lysozyme layer is improved, the water contact angle of the unmodified polyurethane is 73.0 °, while the polyurethane with five bimolecular modified layers The water contact angle of the membrane was 42.3 °. Compared with the unmodified polyurethane, the antibacterial activity of polyurethane modified by layer self-assembly on Staphylococcus aureus was increased by 41.4%. In addition, the surface self-assembled polyurethane The material has better blood compatibility.