【摘 要】
：
Aim: To investigate the antioxidant effects of Morinda officinalis (Morindae radix, MR) on H202-induced oxidative stress in cultured mouse TM3 Leydig cells. Methods: We carded out 2,2-diphenyl-l-picrylhydrazyl free radical scavenging, 3-(4,5-dimethylthiaz
【机 构】
：
Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University, Seou1130-701, K
论文部分内容阅读
Aim: To investigate the antioxidant effects of Morinda officinalis (Morindae radix, MR) on H202-induced oxidative stress in cultured mouse TM3 Leydig cells. Methods: We carded out 2,2-diphenyl-l-picrylhydrazyl free radical scavenging, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase (SOD), and catalase (CAT) assays in Leydig TM3 cells. Results: MR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxi- dative stress was induced in TM3 cells with 100 μmol H2O2, and treatment of the cells with 250 μg/mL MR showed the most significant protective effect (64%, P < 0.001) in the cell viability assay with a decreased lipid peroxidation level (1.75 nmol/mg protein, P < 0.05), increased testosterone production (43.5 pg/mL), and improvements in SOD activity (7.49 units of SOD/mg protein, P < 0.001) and CAT activity (74.6 units of CAT/mg protein, P < 0.001). Conclusion: These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H2O2-induced oxidative stress. (Asian J Androl 2008 Jul; 10: 667-674)
其他文献
Aim: To explore the mechanism of chronic renal failure (CRF)-related erectile dysfunction (ED). Methods: CRF experimental models were established by 5/6 nephrectomy from male Sprague-Dawley rats. Both the rats from the control group (NCRF group, n = 6) an
Aim: To evaluate the relaxant effect of verapamil on human corpus cavernosum in vitro and to assess the drug\'s potential as a treatment for erectile dysfunction (ED). Methods: Preparations of the human corpus cavernosum were obtained from recently dece
Aim: To evaluate the genetic instability of 11 fertile and 25 infertile men. Methods: The methodology of sister chromatid exchanges (SCEs) was applied to cultures of peripheral blood lymphocytes, and the levels of SCEss were analyzed as a quantitative ind
Dear Editor,rnWe recently encountered a rare case of testicular mixed germ cell tumor (MGCT) in a 32-year-old man. The tumor was composed of a combination of a yolk sac tumor, teratoma and rhabdomyosarcomatous somatic type malignancy.
Aim: To determine the involvement of apoptotic cell death in postnatal pathogenesis in mutant strain of hypogonadic (hgn/hgn) rats testes. We evaluated the numbers and types of cells undergoing apoptotic cell death. Methods: Tissue sections were stained b
Aim: To characterize the coexpression of survivin, an inhibitor of apoptosis (IAF), and human telomerase reverse transcriptase (hTERT) in human testes with varying spermatogenic function. Methods: Transcript levels of survivin mRNA and hTERT mRNA were det
Dear Editor,rnI am Dr Hans-Gert Bernstein from the Department of Psychiatry, University of Magdeburg, Germany.Although it is now a well-established fact that mothers with schizophrenia are at higher risk for obstetric complications (preterm births, preecl
Aim: To report two cases of the left testicular artery arching over the left renal vein (LRV) before running downward to the testis. Methods: The subjects were obtained from two Japanese cadavers. During the student course of gross-anatomical dissection,
Various different objects have been reported to strangulate the penis. We reported on a patient who used metal radiator clamps for an extended period of time. Workup included history, physical examination and urinalysis. The patient was taken to the opera