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目的观察体外使用参附注射液对胃肠道恶性肿瘤患者外周血T淋巴辅助(Th)细胞分化的影响。方法 20例拟行择期胃肠道恶性肿瘤手术的患者,麻醉前及手术后24 h抽取外周血分离单个核细胞(PBMC),随机分为两组,分别在培养体系中加入参附注射液(S组)或加入相同体积的生理盐水(C组)。24 h后加入刺激剂植物血凝素(PHA)促进细胞分化。48 h后分析Thl和Th2细胞的比例,测定细胞培养上清液中白细胞介素4(IL-4)和γ干扰素(IFN-γ)浓度。结果手术应激促进Th细胞向Th2方向分化,细胞培养上清液中IFN-γ/IL-4比率降低;参附注射液抑制手术后Th淋巴细胞向Th2方向分化,并改善细胞培养上清液中IFN-γ/IL-4比例。结论参附注射液抑制胃肠道恶性肿瘤患者的Th细胞向Th2方向分化。
Objective To observe the effect of Shenfu injection on differentiation of T lymphocyte (Th) cells in peripheral blood of patients with gastrointestinal cancer in vitro. Methods Twenty patients underwent surgery for elective gastrointestinal cancer. Peripheral blood mononuclear cells (PBMCs) were collected 24 hours before anesthesia and after surgery, and were randomly divided into two groups. Each group was given Shenfu injection S group) or added the same volume of saline (C group). After 24 h, the stimulator phytohemagglutinin (PHA) was added to promote cell differentiation. After 48 h, the ratios of Thl and Th2 cells were analyzed and the concentrations of interleukin 4 (IL-4) and interferon-gamma (IFN-γ) in the cell culture supernatant were determined. Results Surgical stress promoted the differentiation of Th cells to Th2 and decreased the ratio of IFN-γ / IL-4 in the cell culture supernatant. Shenfu injection inhibited the differentiation of Th lymphocytes to Th2 and improved the cell culture supernatant IFN-γ / IL-4 ratio. Conclusion Shenfu injection can inhibit the differentiation of Th cells to Th2 in patients with gastrointestinal cancer.