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目的研究颅内未破裂动脉瘤患者外周血中内皮祖细胞(EPCs)数量、功能及其相关调控因子基质细胞衍化因子-1α(SDF-1α)的变化。方法选择颅内未破裂动脉瘤患者25例和正常对照者20例,以CD34、CD133和KDR阳性细胞作为内皮祖细胞的标记,利用流式细胞仪对外周血EPCs的数量进行检测;同时采用密度梯度离心法收集外周血单个核细胞,通过黏附能力测定实验及Transwell实验检测EPCs的黏附和迁移能力;并以ELISA法对外周血中的SDF-1α水平进行测定。结果颅内未破裂动脉瘤患者较正常对照组外周血中EPCs数量(32.4±3.5 vs 71.8±5.4)、细胞黏附数量(26.5±7.8 vs 76.4±7.5)、迁移数量(12.0±2.9 vs 23.6±6.5)及SDF-1α浓度[(2.323±0.352)ng/mL vs (2.563±0.404)ng/mL]均明显降低,差异均有统计学意义(P<0.05)。结论颅内未破裂动脉瘤患者外周血中EPCs数目降低,且黏附、迁移能力受损,可能与颅内动脉瘤的发生有关。
Objective To study the changes of the number and function of endothelial progenitor cells (EPCs) and the related regulatory factor stromal cell-derived factor-1α (SDF-1α) in peripheral blood of patients with intracranial unruptured aneurysms. Methods Twenty-five patients with intracranial unruptured aneurysms and 20 healthy controls were enrolled in this study. The numbers of EPCs in peripheral blood were detected by flow cytometry using CD34, CD133 and KDR positive cells as markers of endothelial progenitor cells. Density Peripheral blood mononuclear cells were collected by gradient centrifugation. The adhesion and migration ability of EPCs were detected by adhesion assay and Transwell assay. The level of SDF-1α in peripheral blood was determined by ELISA. Results The number of EPCs (32.4 ± 3.5 vs 71.8 ± 5.4), cell adhesion number (26.5 ± 7.8 vs 76.4 ± 7.5), number of migrations (12.0 ± 2.9 vs 23.6 ± 6.5) in the patients with intracranial unruptured aneurysms ) And SDF-1α (2.323 ± 0.352 ng / mL vs 2.563 ± 0.404 ng / mL, respectively). Conclusion The number of EPCs in the peripheral blood of patients with intracranial unruptured aneurysms is decreased, and the adhesion and migration ability is impaired, which may be related to the occurrence of intracranial aneurysms.