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[Objective]To compare the content differences and distribution situation of paeoniflorin and albiflorin contents in different germplasms and parts of Paeonia lactiflora Pall. by using the same analysis method.[Method]HPLC method was adopted. Chromatographic column was LichroCART RP-C 18 (4 mm × 250 mm,5 μm);mobile phase was acetonitrile 0.1% phosphoric acid (14∶86,V/V); flow rate was 1 mL/min;detection wavelength was 230 nm; column temperature was 30 ℃ and injection volume was 10 μL.[Result]There were great differences in the paeoniflorin and albiflorin contents of different germplasms of P.lactiflora.The paeoniflorin contents in different underground parts of P. lactiflora were in the order of rhizome>fibrous root>main root; and the albiflorin contents were in the order of fibrous root>rhizome>main root. [Conclusion]The main factor affecting the contents of paeoniflorin and albiflorin was germplasm.And the paeoniflorin and albiflorin contents in RADIX PAEONIAE ALBA had small variation in authentic producing areas.
[Objective] To compare the content differences and distribution situation of paeoniflorin and albiflorin contents in different germplasms and parts of Paeonia lactiflora Pall. By using the same analysis method. [Method] HPLC method was applied. Chromatographic column was LichroCART RP-C 18 4 mm × 250 mm, 5 μm); mobile phase was acetonitrile 0.1% phosphoric acid (14:86, V / V); flow rate was 1 mL / min; was 10 μL. [Result] There were great differences in the paeoniflorin and albiflorin contents of different germplasms of P. lactiflora. paeoniflorin contents in different underground parts of P. lactiflora were in the order of rhizome> fibrous root> main root; and the albiflorin contents were in the order of fibrous root> rhizome> main root. [Conclusion] The main factor affecting the contents of paeoniflorin and albiflorin was germplasm. And the paeoniflorin and albiflorin contents in RADIX PAEONIAE ALBA had small va riation in authentic producing areas.