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目的观察不同强度恒磁场对糖基化终产物作用下人脐静脉内皮细胞一氧化氮生成及一氧化氮合酶活性的影响。方法采用体外培养第3代人脐静脉内皮细胞,糖孵育法制备糖基化终产物修饰牛血清白蛋白。实验分为6组,即对照组、糖基化终产物修饰牛血清白蛋白(100μg/L)组及糖基化终产物修饰牛血清白蛋白+不同强度(1×10-4T、5×10-4T、10×10-4T2、0×10-4T)磁场组。各组细胞于培养及磁场作用24 h后收集标本,用Griess法检测培养上清中一氧化氮水平,并测定人脐静脉内皮细胞的一氧化氮合酶活性。结果糖基化终产物修饰牛血清白蛋白组一氧化氮含量和一氧化氮合酶活性较对照组明显降低(P<0.05)。1×10-4T、5×10-4T、10×10-4T和20×10-4T恒磁场组一氧化氮生成、一氧化氮合酶活性显著高于糖基化终产物修饰牛血清白蛋白组(P<0.05)。结论糖基化终产物修饰牛血清白蛋白可抑制人脐静脉内皮细胞的一氧化氮合酶活性和一氧化氮产生;1×10-4T~20×10-4T的恒磁场可呈强度依赖性拮抗糖基化终产物修饰牛血清白蛋白对一氧化氮生成的抑制效应。
Objective To observe the effects of different intensities of constant magnetic field on the production of nitric oxide and the activity of nitric oxide synthase in human umbilical vein endothelial cells under the action of advanced glycation end products. Methods The third generation of human umbilical vein endothelial cells were cultured in vitro. Glycation end products were modified by glucose to modify bovine serum albumin. The experiment was divided into 6 groups: control group, advanced glycation end product modified bovine serum albumin (100μg / L) group and advanced glycation end product modified bovine serum albumin + different intensity (1 × 10-4T, 5 × 10 -4T, 10 × 10 -4 T 2, 0 × 10 -4 T) field group. The cells were collected 24 h after culture and magnetic field. The levels of nitric oxide in culture supernatant were detected by Griess method and the activity of nitric oxide synthase in human umbilical vein endothelial cells was determined. Results The levels of nitric oxide and nitric oxide synthase in modified BSA-modified bovine serum albumin were significantly lower than those in control group (P <0.05). 1 × 10-4T, 5 × 10-4T, 10 × 10-4T and 20 × 10-4T constant magnetic field nitric oxide production, nitric oxide synthase activity was significantly higher than the final glycation end products modified bovine serum albumin Group (P <0.05). Conclusion Glycosylation end products modified bovine serum albumin can inhibit nitric oxide synthase activity and nitric oxide production in human umbilical vein endothelial cells; 1 × 10-4T ~ 20 × 10-4T constant magnetic field can be intensity-dependent Inhibitory effect of advanced glycation end products modified bovine serum albumin on nitric oxide production.