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为观察新肝炎病毒TTV在各类高危人群中的感染状况和基因分型 ,在日本株TTVORF1保守区合成了特异性引物 ,采用巢式聚合酶链反应 (nPCR)两次扩增血清TTVDNA ,对各类人群中TTVDNA分别进行了分子克隆和部分基因测序 ,并与日本报道的TTVDNA基因序列比较。结果显示 :从非甲 戊型和非庚型肝炎病人、血清HBsAg阳性的肝炎病人、正常献血员、静脉内吸毒者和女性性乱者中 ,分别获得的 6份TTVDNA克隆 ,其基因序列与日本株TTVORF1部分基因核苷酸序列同源性为 97%～ 99% ,均属于TTVla型。提示 :我国各类高危人群感染TTV以la型为主 ;TTV基因型与疾病发生和传播方式关系不大。
To observe the infection status and genotyping of new hepatitis virus TTV in various high-risk groups, specific primers were synthesized in the conserved region of TTVORF1 strain in Japan and the serum TTVDNA was amplified twice by nested polymerase chain reaction (nPCR) TTVDNA of all kinds of people were molecular cloning and partial gene sequencing, and compared with the TTVDNA gene sequence reported in Japan. The results showed that six of the TTVDNA clones obtained from non-E and non-HepG hepatitis patients, serum HBsAg-positive hepatitis patients, normal blood donors, intravenous drug users and female sexual promiscuity, respectively, The homology of the nucleotide sequence of TTVORF1 gene was 97% -99%, belonging to TTVla type. Tip: all types of high-risk population in China infected with TTV to la type; TTV genotype and disease incidence and mode of transmission is not.