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目的 从孕妇外周血中分离胎儿有核红细胞 (NRBCs) ,并对富集的方法进行比较 ,探讨利用此法进行无创性产前诊断的可行性。 方法 对 5 7例 5～ 40孕周的妇女外周血分别通过单密度梯度离心、免疫细胞化学检测、以及在此基础上流式细胞术分选等三种方法 ,富集 NRBCs。 结果 密度梯度离心后的 2 7例样本中 ,14例观察到 1～ 10个 NRBCs,占 5 1.85 %。免疫细胞化学检测到 CD71 +细胞。流式细胞仪分选出包含 NRBCs的 CD71 + 细胞 ,分选率为 (0 .44± 0 .39)× 10 - 2 。 结论 孕妇外周血中存在胎儿 NRBCs,可通过不同的富集方法获得 ;密度梯度离心法在产前诊断中更具优越性。
Objective To isolate fetal nucleated erythrocytes (NRBCs) from peripheral blood of pregnant women and compare the enrichment methods to explore the feasibility of using this method for noninvasive prenatal diagnosis. Methods Peripheral blood from 5 to 40 gestational weeks in 57 women was enriched in NRBCs by single density gradient centrifugation, immunocytochemistry and flow cytometry based on these methods. Results In 27 samples after density gradient centrifugation, 1 to 10 NRBCs were observed in 14 cases, accounting for 5 1.85%. CD71 + cells were detected by immunocytochemistry. CD71 + cells containing NRBCs were sorted by flow cytometry. The sorting rate was (0.44 ± 0.39) × 10 - 2. Conclusion There are fetal NRBCs in peripheral blood of pregnant women, which can be obtained by different enrichment methods. Density gradient centrifugation has more advantages in prenatal diagnosis.