论文部分内容阅读
目的探讨神经纤维丝蛋白(NF)、神经烯醇化酶(NSE)和突触素(SYN)在人胚胎舌组织不同发育阶段的分布特征。方法应用免疫组织化学法,检测第2~4个月胎龄段共16份人胚胎舌组织内NF、NSE和SYN蛋白的表达,分析其变化规律。结果第2~4个月龄段,NF、NSE和SYN蛋白在人胚舌组织内均有阳性表达。随着胎龄的增大,NF、NSE和SYN在舌组织内阳性表达数量增多,表达强度逐渐增强。第2个月龄时,NF、NSE和SYN蛋白呈少量弱阳性表达,阳性表达强度值分别是135.83±24.62、136.57±15.23和139.84±21.40。第3个月龄时,NF、NSE和SYN阳性表达强度值分别是96.04±23.37、94.89±22.52和90.65±21.08。第4个月胎龄时,NF、NSE和SYN阳性表达强度值分别是79.02±20.90、76.78±21.27和83.43±25.90。应用One-Way ANOVA和LSD-t统计学方法,分析第2~4个月龄段人胚胎舌组织内NF、NSE和SYN蛋白的各自阳性表达强度值,P<0.01。结论第2~4个月龄段,人胚胎舌组织内NF、NSE和SYN的表达强度值随胎龄增大而降低,它们均参与调控人胚胎舌内神经系统和舌肌的分化发育。
Objective To investigate the distribution of neurofilament protein (NF), neuroenenolase (NSE) and synaptophysin (SYN) in different developmental stages of human embryonic tongue tissues. Methods Immunohistochemical method was used to detect the expression of NF, NSE and SYN proteins in 16 human embryonic tongue tissues from the second to the fourth month of gestation, and the changes were analyzed. Results The expression of NF, NSE and SYN was positive in human tongue embryos from the 2nd to 4th month of age. With the increase of gestational age, the positive expression of NF, NSE and SYN increased in the tongue tissue, the expression intensity gradually increased. At 2 months of age, NF, NSE and SYN proteins were slightly weak positive expression, the positive expression intensity values were 135.83 ± 24.62,136.57 ± 15.23 and 139.84 ± 21.40. At 3 months of age, the positive expressions of NF, NSE and SYN were 96.04 ± 23.37, 94.89 ± 22.52 and 90.65 ± 21.08, respectively. The positive expression intensity of NF, NSE and SYN at the 4th month of gestational age were 79.02 ± 20.90, 76.78 ± 21.27 and 83.43 ± 25.90, respectively. One-Way ANOVA and LSD-t statistical methods were used to analyze the positive expressions of NF, NSE and SYN proteins in tongue tissues of the second to fourth months of age, respectively, P <0.01. Conclusions The expressions of NF, NSE and SYN in human embryonic tongue tissues from the second to the fourth month of age are decreased with the increase of gestational age. Both of them are involved in the regulation of the differentiation and development of the neural and lingual muscles in human tongue.