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AIM:To detect the expression of CD44 correlated withthe ability of micro-metastasis in peripheral blood andbone marrow of patients with gastric cancer and to de-duce its clinical significance.METHODS:Preoperative peripheral blood and bonemarrow specimens from 46 patients with gastric cancerand 6 controls were studied by semi-quantitative RT-PCR amplification of CD44v6mRNA.Preoperative andpostoperative peripheral blood specimens from 40patients with gastric cancer and 14 controls were studiedby quantitative RT-PCR amplification of CD44v6rnRNA inthe corresponding period.RESULTS:Semi-quantitative RI-PCR amplificationshowed that CD44v6mRNA expression of peripheralblood and bone marrow was positive in 39 (84.8%)and 40 (86.9%) of 46 patients with gastric cancer,respectively.In peripheral blood,CD44v6mRNAexpression was positive for diffuse type in 30 (93.8%)of 32 patients and for intestinal type in 9 (64.3%)of 14 patients.On the other hand,in bone marrow,CD44v6mRNA expression was positive for diffuse typein 31 (96.9%) of 32 patients and for intestinal typein 10 (71.4%) of 14 patients.There was a significantdifference between the diffuse type and intestinal type.Quantitative RT-PCR amplification demonstrated thatCD44v6mRNA was not expressed in the peripheral bloodof controls and CD44v6mRNA expression was positivefor preoperative peripheral blood in 40 patients withgastric cancer,the expression levels being from 4.9×102 to 3.2×10~5 copies/g RNA.The average expressionlevel of CD44v6mRNA in peripheral blood was 3.9×10~(10) copies/g RNA.The expression levels of CD44v6mRNAin peripheral blood in gastric cancer patients aftercurative operation increased from 5.5×100 to 7.6×10copies/g RNA (P=0.00496).After curative operation,theexpression level decreased markedly.CONCLUSION:Semi-quantitative and quantitative RT-PCR amplification for CD44v6mRNA is a sensitive andspecific method for the detection of micro-metastasis inperipheral blood and bone marrow,which might be usedas an indicator of tumor burden and therapeutic effect.
AIM: To detect the expression of CD44 correlated with the ability of micro-metastasis in peripheral blood and bone marrow of patients with gastric cancer and to de-duce its clinical significance. METHODS: Preoperative peripheral blood and bonemarrow specimens from 46 patients with gastric cancerand 6 controls were studied by semi-quantitative RT-PCR amplification of CD44v6 mRNA. Preoperative and postoperative peripheral blood specimens from 40 patients with gastric cancer and 14 controls were studied by quantitative RT-PCR amplification of CD44v6rnRNA inthe corresponding period .RESULTS: Semi-quantitative RI-PCR amplifications demonstrated that CD44v6 mRNA expression of peripheral blood and bone marrow was positive in 39 (84.8%) and 40 (86.9%) of 46 patients with gastric cancer, respectively.In peripheral blood, CD44v6 mRNA expression was positive for diffuse type in 30 (93.8%) of 32 patients and for intestinal type in 9 (64.3%) of 14 patients. Of the other hand, in bone marrow, CD44v6 mRNA expression was positive for diffuse typein 31 (96.9%) of 32 patients and for intestinal typein 10 (71.4%) of 14 patients. There was a significant difference between the diffuse type and intestinal type. Quantitative RT-PCR amplification demonstrated that CD44v6 mRNA was not expressed in the peripheral blood of controls and CD44v6 mRNA expression was positive for preoperative peripheral blood in 40 patients with gastric cancer, the expression levels being from 4.9 × 102 to 3.2 × 10 ~ 5 copies / g RNA. The average expression level of CD44v6 mRNA in peripheral blood was 3.9 × 10 ~ (10) copies / g RNA.The expression levels of CD44v6 mRNA in peripheral blood in gastric cancer patients after curative operation increased from 5.5 × 100 to 7.6 × 10copies / g RNA (P = 0.00496). After curative operation, theexpression level decreased markedly. CONCLUSION: Semi-quantitative and quantitative RT-PCR amplification for CD44v6 mRNA is a sensitive and specific method for the detection of micro-metastasis inperipheral blood and bone marrow, which might be usedas ind icator of tumor burden and therapeutic effect.