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目的：探讨将外周血单个核细胞（ＰＢＭＮＣ）的促血浆凝固活性（ＰＣＡ）用于急性排斥反应（ＡＧＲ）监测的可能性。方法：将新西兰白兔随机分为３组：第１组做自体皮肤移植（８只），第２、３组做异体皮肤移植（８、３只），第３组于术前１ｄ至术后７ｄ皮下注射环孢霉素Ａ５ｍｇ／（ｋｇ·ｄ）。术后每天观察移植皮片的变化。术前１ｄ及术后隔日取耳缘静脉血测ＰＣＡ和激活淋巴细胞计数，同时取移植皮肤组织作病理检查。结果：同种异体皮肤移植动物在出现ＡＧＲ前，其ＰＢＭＮＣ离体后不经培养即表现较强的促凝活性（ＳＰＣＡ），经供体抗原刺激后的ＰＣＡ（ＤＡｇＰＣＡ）活性也于ＡＧＲ前开始增强，而且表明具有抗原特异性。结论：ＰＣＡ检测方法在ＡＧＲ早期监测方面有一定的应用前景。
Objective: To investigate the possibility of using peripheral blood mononuclear cells (PBMNC) for promoting plasma coagulation activity (PCA) for acute rejection (AGR) monitoring. Methods: New Zealand white rabbits were randomly divided into 3 groups: Group 1 received autologous skin grafts (8), Groups 2 and 3 received allogeneic skin grafts (8, 3), group 3 7d subcutaneous injection of cyclosporine A5mg / (kg · d). The changes of transplanted skin were observed daily. Preoperative and postoperative day after taking the ear vein blood of PCA and activation of lymphocyte count, while taking the skin graft for pathological examination. RESULTS: Before AGR was observed, PBMNC showed strong pro-coagulant activity (SPCA) without culture, PCA (DAgPCA) activity stimulated by donor antigen also started before AGR Enhanced, but also showed antigen specificity. Conclusion: The PCA detection method has certain application prospects in the early monitoring of AGR.