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目的建立基质辅助激光解吸电离飞行时间质谱技术(MASSARRAY)测定中国肾移植人群的CYP3A5*3、CYP3AP1和多药耐药基因(MDR1)的多态性。方法用MASSARRAY技术对60例中国肾移植人群的CYP3A5*3、CYP3AP1和MDR1位点进行基因分型。结果 CYP3A5*1等位基因占25.9%,*3等位基因占74.1%,CYP3AP1A等位基因占26.7%,G等位基因占73.3%,MDR1C1236T、G2677T/A、C3435T个体突变频率分别为65.6%、38.3%、36.6%。结论中国肾移植人群中CYP3A5*3、CYP3AP1和MDR1位点存在遗传多态性,影响不同个体药物代谢酶活性和药物代谢。
Objective To establish a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MASSARRAY) for the determination of CYP3A5 * 3, CYP3AP1 and multidrug resistance (MDR1) polymorphisms in Chinese renal transplant recipients. Methods MASSARRAY technique was used to genotype CYP3A5 * 3, CYP3AP1 and MDR1 in 60 Chinese kidney transplant recipients. Results CYP3A5 * 1 allele accounted for 25.9%, * 3 allele 74.1%, CYP3AP1A allele 26.7%, G allele 73.3%, MDR1C1236T, G2677T / A, C3435T individuals were 65.6% , 38.3%, 36.6%. Conclusion There are genetic polymorphisms in CYP3A5 * 3, CYP3AP1 and MDR1 loci in renal transplant recipients in China, which may affect the activity of drug metabolizing enzymes and drug metabolism in different individuals.