论文部分内容阅读
牡丹皮为毛莨科芍药属牡丹(Paeonia suffruticosa Andr)的根皮;徐长卿为萝藦科牛皮消属植物徐长卿(Cynanchum paniculatum (Bunge) Kitag)的根及全草,是常用的两种具有抗菌消炎的中草药,其主要有效成分均含有丹皮酚(Patonol).多以清热凉血、活血化瘀、祛风化湿、跌扑损伤等功能应用于临床。药典规定有效成分丹皮酚的含量用分光光度法进行测定,文献报道亦有用容量法、库仑法、薄层扫描法进行测定,用现代胶束荧光分析技术进行含量测定的未见报道。本文在8种表面活性物质中经过筛选实验,使用十二烷基硫酸钠(SDS)为胶束试剂,利用SDS在水溶液中亲水基团向外,疏水基团向里的有序排列,当浓度达到或超过一定浓度(临界浓度CMC)时形成胶束,在胶束的胶核里是一个疏水的有机环境,有利于丹皮酚的溶
Paeonia suffruticosa Andr is the root bark of Paeonia suffruticosa Andr. It is the root and whole plant of Cynanchum paniculatum (Bunge) Kitag. It is commonly used and has two types of anti-bacterial and anti-inflammatory agents. The main active ingredients of Chinese herbal medicines contain Patonol. Many of them are used for clinical purposes such as heat and cooling blood, blood circulation, dehumidification, dampness, and puff damage. The pharmacopoeia stipulates that the content of paeonol is determined by spectrophotometry. The literature reports are also measured by the volumetric method, coulometric method, and thin-layer scanning method. There are no reports on the determination of paeonol by modern micelle fluorescence analysis techniques. In this article, eight kinds of surface active substances were subjected to screening experiments using sodium dodecyl sulfate (SDS) as the micelle reagent, and SDS was used to arrange the hydrophilic groups outward and the hydrophobic groups inward in the aqueous solution. The micelles are formed when the concentration reaches or exceeds a certain concentration (critical concentration CMC), which is a hydrophobic organic environment in the colloidal micelles, which is beneficial to the dissolution of paeonol.