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为了解SEN病毒中国株的流行病学及基因序列 ,采集肝炎患者 (甲～戊型 )、非甲～非戊型肝炎患者、ALT升高的婴幼儿、尿毒症患者、静脉毒瘾者和健康体检人群的血清标本 ,用巢式PCR检测SEN病毒 ,结果他们的检出率依次为 13 3%、2 0 5 %、4 8%、5 8%、5 7%和 0。PCR获得了两种不同长度阳性片段。选取 5份阳性PCR产物进行纯化、连接及转化 ,获取重组克隆 ,命名为 pGCEM -SENVL(1、2、3)、pGEM -SENVS(1、2 ) ,进行DNA序列分析。结果与SEN病毒 (AX0 2 5 6 6 7)序列同源性分别为 87 7%、76 1%、88 2 %、72 7%和 78 8%。首次发现我国存在SEN病毒散发感染 ,并存在不同于SEN病毒 (AX0 2 5 6 6 7)的中国变异株 ,而且有同一患者存在两种变异株的复合感染
In order to understand the epidemiology and gene sequence of SEN virus in China, hepatitis patients (A ~ E), non-A to non-E hepatitis, infants with elevated ALT, uremic patients, intravenous drug users and healthy SEN virus was detected by nested PCR in the sera from the physical examination population. The detection rate was 113%, 25%, 48%, 58%, 57% and 0 respectively. PCR obtained two different lengths of positive fragments. Five positive PCR products were selected for purification, ligation and transformation, and the recombinant clones were obtained and named as pGCEM-SENVL (1,2,3), pGEM-SENVS (1,2) for DNA sequence analysis. Results The sequence homologies with the SEN virus (AX0 2 5 6 6 7) were 87 7%, 76 1%, 88 2%, 72 7% and 78 8%, respectively. For the first time, it was found that SEN virus was present in China and there was a Chinese variant strain different from that of SEN virus (AX0256667), and there was a combination of two variants in the same patient