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目的:研究miRNA-497在上皮性卵巢癌组织/细胞中的表达情况,miRNA-497对脂肪酸合成酶(fatty acid synthetase,FASN)表达水平的影响,探讨miRNA-497与FASN之间的关系,为临床卵巢癌诊治以及选择性靶向药物应用提供新的实验研究依据。方法:q RT-PCR分析10例上皮性卵巢癌组织及10例正常上皮性卵巢组织中miRNA-497、FASN的表达水平,Person相关性分析二者表达水平有无相关性。从卵巢癌细胞株SKOV3、A2780中筛选出miRNA-497表达量较低的SKOV3细胞株,转染miRNA-497 mimics、miRNA-497 inhibitor至卵巢癌细胞株SKOV3中,转染miRNA-497 mimics control,miRNA-497 inhibitor control作为阴性对照,未转染组为空白对照,q RT-PCR检测不同组中FASN的m RNA的表达情况,Western blot分析各组细胞中FASN蛋白表达改变情况。结果:1miRNA-497在上皮性卵巢癌组织中的表达较正常卵巢组织明显降低,而FASN基因却存在异常高表达;Pearson相关性分析表明,卵巢癌组织和正常卵巢组织中miR-497与FASN m RNA表达存在负相关(r=-0.458 84,P<0.01);2miRNA-497可以调控FASN的表达。与对照组相比,增加miRNA-497的表达后FASN的蛋白及m RNA表达水平明显降低;抑制miNRA-497的表达可增加FASN蛋白及m RNA的表达。结论:miRNA-497在上皮性卵巢癌组织中的表达较正常卵巢组织明显降低,而FASN基因却存在异常高表达,二者表达水平存在负相关;miRNA-497能够抑制卵巢癌SKOV3细胞中FASN的表达,FASN可能是miRNA-497的靶基因。
OBJECTIVE: To investigate the expression of miRNA-497 in epithelial ovarian cancer tissues / cells and the effect of miRNA-497 on the expression of fatty acid synthetase (FASN), and to explore the relationship between miRNA-497 and FASN. Clinical diagnosis and treatment of ovarian cancer and selective targeted drug applications provide a new experimental basis for the study. Methods: q RT-PCR was used to analyze the expression of miRNA-497 and FASN in 10 cases of epithelial ovarian cancer and 10 cases of normal epithelial ovarian tissue. Correlation analysis was made between the two expression levels of miRNA-497 and FASN. MiRNA-497 mimics and miRNA-497 inhibitor were transfected into SKOV3 ovarian cancer cell line SKOV3, and miRNA-497 mimics control was transfected into SKOV3 cell line with low expression of miRNA-497 from ovarian cancer cell lines SKOV3 and A2780. miRNA-497 inhibitor control as a negative control, untransfected group as a blank control, qRT-PCR detection of different groups of FASN m RNA expression, Western blot analysis of each group of cells FASN protein expression changes. Results: The expression of 1miRNA-497 in epithelial ovarian cancer was significantly lower than that in normal ovarian tissue, while the expression of FASN gene was abnormally high. Pearson correlation analysis showed that the expression of miR-497 and FASN m in ovarian cancer tissue and normal ovarian tissue RNA expression was negatively correlated (r = -0.458 84, P <0.01); 2miRNA-497 could regulate the expression of FASN. Compared with the control group, the expression of miRNA-497 increased significantly after FASN protein and m RNA expression; inhibition of miNRA-497 expression increased FASN protein and m RNA expression. Conclusions: The expression of miRNA-497 in epithelial ovarian cancer tissues is significantly lower than that in normal ovarian tissues, while the expression of FASN gene is abnormally high, and the expression of miRNA-497 is negatively correlated. MiRNA-497 can inhibit the expression of FASN in ovarian cancer SKOV3 cells FASN may be the target gene of miRNA-497.