论文部分内容阅读
为探讨HSV－TK基因介导的GCV系统对人视网膜母细胞瘤（RB）基因治疗的有效性，利用电穿孔技术把重组逆转录病毒载体pLXSNTK导入包装细胞PA317，筛选出逆转录病毒产生细胞PA317/TK，收获病毒。体外实验：用逆转录病毒感染RB细胞，筛选出含TK基因的RB细胞（RB/TK），用GCV分别处理RB、RB/TK及不同比例的混合细胞。体内实验：建立人RB裸鼠原位异种移植模型，瘤内注射病毒液后再经GCV处理。结果：体外实验RB/TK细胞对GCV的敏感性显著高于RB细胞，并存在旁观者效应；RB荷瘤裸鼠内TK/GCV系统对RB细胞的生长也有明显的抑制作用。提示HSVTK/GCV系统有望成为人RB肿瘤的基因治疗途径。
To investigate the efficacy of HSV-TK gene-mediated GCV system for human retinoblastoma (RB) gene therapy, the recombinant retroviral vector pLXSNTK was introduced into packaging cell PA317 by electroporation, and retrovirus-producing cells PA317 were selected. /TK, harvest the virus. In vitro: RB cells were infected with retrovirus, RB cells (RB/TK) containing TK gene were screened, and RB, RB/TK and mixed cells of different proportions were treated with GCV. In vivo experiment: An in situ xenograft model of human RB nude mice was established. The virus solution was injected intratumorally and then treated with GCV. RESULTS: The sensitivity of RB/TK cells to GCV in vitro was significantly higher than that of RB cells, and there was a bystander effect. The TK/GCV system in RB-bearing nude mice also significantly inhibited the growth of RB cells. The HSVTK/GCV system is expected to become a gene therapy approach for human RB tumors.