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目的通过检测梅毒血清固定患者外周血单个核细胞(PBMC)中Toll样受体4 mRNA(TLR4 mRNA)的表达水平,探讨其与梅毒血清固定的关系。方法选取梅毒血清固定患者40例为观察组,采用实时定量荧光(Real-time PCR)方法检测外周血TLR4 mRNA的表达水平,设40例健康者作为对照1,设经正规驱梅治疗后快速血浆反应素环状卡片试验(RPR)转阴者作为对照组2。结果梅毒血清固定组外周血中TLR4 mRNA的表达水平明显低于健康对照组及RPR转阴组,差异均有统计学意义(P<0.05)。结论梅毒血清固定患者外周血Toll样受体4 mRNA的表达水平明显低于健康人群及经正规驱梅治疗后RPR转阴者,表明Toll样受体4 mRNA的表达异常可能与梅毒血清固定的发生有一定联系。
Objective To detect the expression of TLR4 mRNA (TLR4 mRNA) in peripheral blood mononuclear cells (PBMCs) from patients with syphilis and to explore its relationship with syphilis serum. Methods Forty patients with syphilis serum were selected as observation group. Real-time quantitative PCR (Real-time PCR) was used to detect the expression of TLR4 mRNA in peripheral blood. Forty healthy people as control 1, Receptor cyclic card test (RPR) negative as control group 2. Results The expression of TLR4 mRNA in peripheral blood of syphilis serum-fixed group was significantly lower than that of healthy control group and RPR-negative group (P <0.05). Conclusion The expression level of Toll-like receptor 4 mRNA in peripheral blood of patients with syphilis is significantly lower than that of healthy people and RPR-negative patients after formalin administration, indicating that the abnormal expression of Toll-like receptor 4 mRNA may be related to the occurrence of syphilis serum fixation Have some contact.