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目的探讨壹期矽肺患者血清中血红素氧合酶-1(HO-1)、脂质过氧化产物(LPO)和γ-干扰素诱导蛋白10(IP-10)水平变化及意义。方法采用酶联免疫吸附法检测62例壹期矽肺(矽肺组)、37例具有与矽肺组相同接尘条件的健康井下接尘矿工(接尘组)和35例非接尘井上健康人员(对照组)血清中HO-1水平;用Luminex流式荧光技术检测血清中IP-10水平;化学比色法检测血清中LPO水平。结果与对照组比较,接尘组血清中LPO水平明显升高,IP-10水平明显降低,差异均有统计学意义(P<0.01),矽肺组血清中LPO和IP-10水平均明显升高,差异均有统计学意义(P<0.01和P<0.05);与接尘组比较,矽肺组血清中HO-1水平显著降低,IP-10水平明显升高,差异均有统计学意义(P<0.05和P<0.01);饮酒矽肺患者血清中HO-1水平低于非饮酒矽肺患者(P<0.05);壹期矽肺患者血清中LPO与IP-10呈负相关(r=-0.407,P值=0.015)。结论壹期矽肺患者血清中HO-1、LPO及IP-10水平异常与矽肺发生发展有关;饮酒可影响壹期矽肺患者血清中HO-1水平。
Objective To investigate the changes and significance of serum HO-1, LPO and IP-10 in patients with stage I silicosis. Methods Sixty-two cases of silicosis (silicosis group I) were detected by enzyme-linked immunosorbent assay (ELISA). Thirty-seven healthy underground dust miners (dust exposure group) and 35 healthy people without dust exposure well Group). The level of IP-10 in serum was detected by Luminex flow cytometry. The level of LPO in serum was detected by chemical colorimetry. Results Compared with the control group, the level of LPO and the level of IP-10 in the dust-exposed group were significantly decreased (P <0.01), and the levels of LPO and IP-10 in the silicosis group were significantly increased (P <0.01 and P <0.05). Compared with the control group, the level of HO-1 in serum and the level of IP-10 in silicosis group were significantly increased (P <0.05 and P <0.01). The serum levels of HO-1 in alcoholic silicosis patients were lower than those in non-alcoholic silicosis patients (P <0.05). LPO and IP-10 in serum of patients with silicosisⅠwas negatively correlated (r = -0.407, P Value = 0.015). Conclusions The abnormal levels of HO-1, LPO and IP-10 in serum of patients with silicosis Ⅰ are related to the development and progression of silicosis. Alcohol consumption may affect the level of HO-1 in the serum of patients with silicosis Ⅰ.