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目的：研究人乳头瘤病毒（ＨＰＶ）与阴茎癌的关系。方法：应用原位ＰＣＲ技术对４６例阴茎癌组织中的ＨＰＶ１６和ＨＰＶ１８ＤＮＡ进行检测。结果：３３例阴茎癌中检测到了ＨＰＶＤＮＡ（７１．７％），其中２９例ＨＰＶ１６ＤＮＡ阳性（６３．０％），６例ＨＰＶ１８ＤＮＡ阳性（１３．０％），２例ＨＰＶ１６和ＨＰＶ１８ＤＮＡ均阳性，４例ＨＰＶ１８ＤＮＡ阳性而ＨＰＶ１６阴性；２例淋巴结转移癌，３例癌旁不典型增生组织和１例癌旁增生组织ＨＰＶ１６ＤＮＡ阳性。结论：阴茎癌与ＨＰＶ１６、ＨＰＶ１８感染有密切关系，原位ＰＣＲ技术是一项敏感性高、特异性强的技术。
Objective: To study the relationship between human papillomavirus (HPV) and penile cancer. Methods: HPV16 and HPV18 DNA were detected in 46 cases of penile cancer by in situ PCR. RESULTS: HPV DNA (71.7%) was detected in 33 cases of penile carcinoma, of which 29 cases were positive for HPV16 DNA (63.0%), 6 cases were positive for HPV18 DNA (13.0%), 2 cases were positive for HPV16 and HPV18 DNA, and 4 cases were positive. HPV18 DNA was positive and HPV16 was negative; 2 cases of lymph node metastases, 3 cases of atypical hyperplastic tissue adjacent to cancer, and 1 case of hyperplasia of HPV16 DNA. Conclusion: Penile cancer is closely related to HPV16 and HPV18 infection. In situ PCR is a highly sensitive and specific technique.