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近年研究表明,镉对胎盘有毒,表现为近足月产孕鼠胎盘坏死和胎仔死亡。本研究的目的,是通过体外试验方法,观察 Cd 对人体胎盘某些与外源性化合物代谢有关的酶,如芳基羟化酶(AHH)(Ⅰ相酶)、苯醌还原酶(OR)和儿茶酚胺-O-甲基转移酶(COMT)(Ⅱ相酶),以及与能量生成有关的葡萄糖-6-磷酸脱氢酶(G-6-PD)活性的影响,评价 Cd 的胎盘毒性。胎盘移植物的制备胎盘来自 Ramban 医学中心妊娠3个月的非吸烟妇女,用消毒的生理盐水冲洗去除血液,再放入含2％抗生素的DMEM 液中冲洗,取50 mg 胎盘于生理盐水中切割,放于6孔的 Corning 平皿中,每孔加含1％抗生素的 DMEM 液2.0 ml,各孔所加DMEM 液的含 Cd 量分别为0、0.75、1.5、3.0、6.0和12 mg/L,37℃孵育6和24 h。在孵育过程中通入95％空气和5％CO_2混合气体。孵育结束后,移出胎盘移植物的肌肉层,以0.25 mol/L 的蔗糖液2.0 ml 制备匀浆,离心,取上清液测定酶活性及蛋白质含量。结果表明,AHH 活性对 Cd 的剂量呈双相反应,低剂量时,两个孵育时间6和24 hAHH
Recent studies have shown that cadmium on the placenta is toxic, manifested as placental necrosis and fetal death in nearly full-term pregnant rats. The purpose of this study was to observe the effects of Cd on some human placental enzymes related to the metabolism of exogenous compounds such as aryl hydroxylase (AHH) (phase I enzyme), benzoquinone reductase (OR) And catecholamine-O-methyltransferase (COMT) (phase II enzyme), and glucose-6-phosphate dehydrogenase (G-6-PD) activity related to energy production. Preparation of Placenta Grafts Placenta from non-smoking women at 3 months of pregnancy at the Ramban Medical Center was washed with sterilized saline to remove the blood, rinsed in DMEM with 2% antibiotics and cut into 50 mg placentas in saline Placed in a 6-well Corning plate and added with 2.0 ml DMEM solution containing 1% antibiotics per well. The Cd content in DMEM solution added to each well was 0, 0.75, 1.5, 3.0, 6.0 and 12 mg / L, Incubate at 37 ° C for 6 and 24 h. During the incubation process, 95% air and 5% CO 2 gas were introduced. After incubation, the muscle layer of the placenta grafts was removed and homogenized with 2.0 ml of 0.25 mol / L sucrose solution, centrifuged, and the supernatant was used to measure the enzyme activity and protein content. The results showed that AHH activity showed a biphasic response to the dose of Cd, and at low doses, the two incubation times were 6 and 24 hAHH