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为了解叶酸防治胃癌可能的分子机理,以放免法观察46例正常人、33例胃癌患者血浆叶酸。分别以~3H-SAM掺入法和Southern blot检测21例进展期胃癌手术标本的癌区、癌旁和外周正常区组织总基因组DNA和癌基因甲基化情况。胃癌患者血浆叶酸水平降低。胃癌区总基因组DNA甲基化水平低于癌旁和外周正常区。49％和62％的癌灶和癌旁的c-myc癌基因片段及50％和40％的癌区和癌旁的c-Ha-ras癌基因片段呈低甲基化;且低甲基化病例血浆叶酸值显著低于正常甲基化者(P<0.05)。叶酸的供甲基作用可能对胃癌的发生发展有一定的影响。
In order to understand the possible molecular mechanisms of folic acid in preventing and treating gastric cancer, plasma folic acid was observed by radioimmunoassay in 46 normal subjects and 33 gastric cancer patients. The methylation status of total genomic DNA and oncogenes in cancer tissues, paracancerous and peripheral normal tissues of 21 advanced gastric cancer specimens were detected by ~3H-SAM incorporation method and Southern blot. In patients with gastric cancer, plasma folic acid levels decreased. The total genomic DNA methylation levels in gastric cancer regions were lower than those in the paracancerous and peripheral normal areas. 49% and 62% of foci and paracancerous c-myc oncogene fragments and 50% and 40% of cancerous regions and paracancerous c-Ha-ras oncogene fragments hypomethylated; and hypomethylated The plasma folate value was significantly lower in patients than in normal methylation (P<0.05). The methylation effect of folic acid may have some influence on the occurrence and development of gastric cancer.