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[Objective] To establish the rapid propagation system from the leaves of Liriope spicata (Thunb.) Lour. var. prolifera Y.T.Ma. [Method] Effects of external hormones on the callus formation, adventitious bud differentiation and proliferation, adventitious root formation were explored after the culture of L. spicata tender leaves as explants in MS basal medium with the addition of 6-BA, NAA and GA. [Result] The callus induction rate of L. spicata leaves was more than 20% in the medium of MS+0.1-2.0 mg/L 6-BA+0.01-0.5 mg/L NAA. In the medium of MS+0.1-1.0 mg/L 6-BA+0.01-0.5 mg/L NAA+0.1-1.0 mg/L GA, the growth and proliferation effects of buds were good, which pointed out that GA could improve the surviving rate of seedlings. The medium of 1/2 MS supplemented with 0.1-0.5 mg/L NAA or 0.1 mg/L IBA or 0.1-0.5 mg/L IAA could make the rooting rate reach 97% during 20 days. If the sprouts were transplanted into the substrate in which the rate between leaf mold and perlite was 5∶ 3, the survival rate could reach over 90%. [Conclusion] The optimum rapid propagation system from leaves was selected, which provided theoretical basis for the development and utilization of L. spicata.
[Objective] To establish the rapid propagation system from the leaves of Liriope spicata (Thunb.) Lour. Var. Prolifera YTMa. [Method] Effects of external hormones on the callus formation, adventitious bud differentiation and proliferation, adventitious root formation were explored after the culture of L. spicata tender leaves as explants in MS basal medium with the addition of 6-BA, NAA and GA. [Result] The callus induction rate of L. spicata leaves was more than 20% in the medium of MS + 0.1-2.0 mg / L 6-BA + 0.01-0.5 mg / L NAA. In the medium of MS + 0.1-1.0 mg / L 6-BA + 0.01-0.5 mg / L NAA + 0.1-1.0 mg / L GA, the growth and proliferation effects of buds were good, which pointed out that GA could improve the surviving rate of seedlings. The medium of 1/2 MS supplemented with 0.1-0.5 mg / L NAA or 0.1 mg / L IBA or 0.1-0.5 mg / L IAA could make the rooting rate reach 97% during 20 days. If the sprouts were transplanted into the substrate in which the rate between leaf mold and perlite was 5: 3, the survi val rate could reach over 90%. [Conclusion] The optimum rapid propagation system from leaves was selected, which provides theoretical basis for the development and utilization of L. spicata.