【摘 要】
：
Background:Genetic selection in cattle has been directed to increase milk production. This, coupled to the fact that the vast majority of bovine artificial inseminations (AI) are performed using cryopreserved sperm, have led to a reduction of fertility ra
【机 构】
：
Biotechnology of Animal and Human Reproduction(TechnoSperm),Faculty of Sciences,University of Girona
论文部分内容阅读
Background:Genetic selection in cattle has been directed to increase milk production. This, coupled to the fact that the vast majority of bovine artificial inseminations (AI) are performed using cryopreserved sperm, have led to a reduction of fertility rates over the years. Thus, seeking sensitive and specific sperm biomarkers able to predict fertility rates is of vital importance to improve cattle reproductive efficiency. In humans, sperm chromatin condensation evaluated through chromomycin A3 (CMA3) has recently been purported to be a powerful biomarker for sperm functional status and male infertility. The objectives of the present study were:a) to set up a flow cytometry method for simultaneously evaluating chromatin condensation and sperm viability, and b) to test whether this parameter could be used as a predictor of in vivo fertility in bulls. The study included pools of three independent cryopreserved ejaculates per bull from 25 Holstein males. Reproductive outcomes of each sire were determined by non-return rates, which were used to classify bulls into two groups (highly fertile and subfertile). Results:Chromatin condensation status of bovine sperm was evaluated through the combination of CMA3 and Yo-Pro-1 staining and flow cytometry. Sperm quality parameters (morphology, viability, total and progressive motility) were also assessed. Pearson correlation coefficients and ROC curves were calculated to assess their capacity to predict in vivo fertility. Sperm morphology, viability and total motility presented an area under the ROC curve (AUC) of 0.54, 0.64 and 0.68, respectively (P>0.05), and thus were not able to discriminate between fertile and subfertile individuals. Alternatively, while the percentage of progressively motile sperm showed a significant predictive value, with an AUC of 0.73 (P=0.05), CMA3/Yo-Pro-1 staining even depicted superior results for the prediction of in vivo fertility in bulls. Specifically, the percentage of viable sperm with poor chromatin condensation showed better accuracy and precision to predict in vivo fertility, with an AUC of 0.78 (P=0.02). Conclusions:Chromatin condensation evaluated through CMA3/Yo-Pro-1 and flow cytometry is defined here as a more powerful tool than conventional sperm parameters to predict bull in vivo fertility, with a potential ability to maximising the efficiency of dairy breeding industry.
其他文献
Mycotoxins are secondary metabolites produced by fungus. Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs. So, powerful detection methods are vital and effective ways to prevent feed contamination. Traditional det
Background:Whey permeate is an effective lactose source for nursery pigs and the most benefits are obtained when pigs are at 7 to 11 kg BW. Altering weaning ages could cause different length of early-weaner phases until 7 kg BW and thus it would influence
Background:Spermatogenesis is the process by which male gametes are formed from spermatogonial stem cells and it is essential for the reliable transmission of genetic information between generations. To date, the dynamic transcriptional changes of defined
Methane from enteric fermentation is the gas with the greatest environmental impact emitted by ruminants. Lovastatin (Lv) addition to feedstocks could be a strategy to mitigate rumen methane emissions via decreasing the population of methanogenic archaea
Background:Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock industry. Concentrations of the small peptide hormone oxytocin (OXT), involved in male reproductive function and present in the seminal plasma (SP
Background:Follicular atresia has been shown to be strongly associated with a low follicle utilization rate and female infertility, which are regulated by many factors such as microRNAs (miRNAs), which constitute a class of noncoding RNAs (ncRNAs). Howeve
对采自西藏不同海拔的3份野生披碱草属Elymus和2份青海引进的披碱草属Elymus材料进行农艺学性状和生产性能评价,为西藏高寒地区筛选育种材料提供理论依据.结果表明:供试的5份披碱草品种均可在拉萨地区进行生产和开发利用.采自高寒草原海拔4 045 m的工布江达披碱草和青海引进的青牧1号老芒麦表现出较好的生产性能,可作为饲草型牧草进行选育;采自海拔4 360 m的当雄披碱草和2种青海引进的披碱草穗数较多,穗子较长,表现出较好的种子产量,其中采自海拔4 360m的当雄披碱草叶量较丰富,适口性好,可作为放牧型
调研日喀则市桑珠孜区、白朗县、拉孜县、江孜县、萨迦县等各县(区)蔬菜生产基地,详细了解了当地蔬菜产业情况,开展了蔬菜科研和技术指导等工作,对日喀则市蔬菜产业进行了积极的探索和研究,详细介绍了 日喀则市发展蔬菜产业的优势、发展现状、存在问题及发展建议,为日喀则市蔬菜产业高质量发展提供理论和实践依据.
草地贪夜蛾Spodoptera frugiperda是一种全球重大迁飞性害虫,于2019年首次入侵我国.在西藏自治区调查草地贪夜蛾发生范围及危害情况过程中,容易将草地贪夜蛾与黏虫、棉铃虫、斜纹夜蛾等夜蛾科近缘种相混淆.为了区分草地贪夜蛾及其他夜蛾科近缘种,从寄主植物、危害状、幼虫特征和成虫特征比较了 4种夜蛾科害虫,并结合资料进行对比区分,综合分析草地贪夜蛾与其他3种鳞翅目害虫的区别,总结了草地贪夜蛾的关键特征,结果显示4种害虫在幼虫时期特征较为明显,容易区分,建议从幼虫时期进行区分.
青稞酒营养丰富,风味独特,深受消费者喜爱.本文首先对青稞酒企业起源以及发展进行了概述,然后从青稞酒企业的注册资本、全国在业的青稞酒企业的分布、涉及的行业以及经营情况等方面分析了青稞酒企业的现状,最后为青稞酒企业的健康发展提出建议,以供参考.