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5 氨基γ 酮戊 (ALA)及其己酯 (He ALA)具有内源生成光敏剂的特点 ,在肿瘤光动力探测及治疗中显示出了优势。ALA及He ALA对神经母细胞瘤、肝癌细胞及成纤维细胞的光动力作用被研究比较。由特征荧光光谱证实 ,经ALA或He ALA培养后 ,三种细胞内均可生成原卟啉 (PpIX)产物。激光扫描荧光显微镜显示 ,在经ALA或He ALA培养后的神经母细胞瘤中 ,PpIX均以弥散方式分布在细胞质中。PpIX在三种细胞中的积聚动力学过程不同 ,随着ALA或He ALA培育时间的增长 ,PpIX在肝癌细胞及成纤维原细胞中的积累增加 ,而在神经母细胞瘤中PpIX在 8h后已达到饱和。此外 ,在同样的培育条件下 ,神经母细胞瘤中PpIX的生成浓度明显高于肝癌细胞及成纤维细胞。经ALA培养及光照射后 ,可使近 90 %的神经母细胞瘤失活 ;而在同样条件下却只能杀伤 5 0 %左右的肝癌细胞及成纤维细胞。揭示了神经母细胞瘤对ALA光动力作用有极高的敏感性 ,并适于光动力治疗。与ALA相比 ,He ALA可在三种细胞内造成与ALA相近的杀伤率 ,但所用的药物浓度却比ALA低 10倍 ,显示He ALA具有极高的光动力灭活效率。因此在内源光动力治疗中 ,He ALA是一种极具开发前景的新药物。
5 Amino-γ-keto-pentanone (ALA) and its hexyl ester (He ALA) have endogenous generation of photosensitizers and have shown their advantages in photodynamic therapy of tumors. The photodynamic effects of ALA and He ALA on neuroblastoma, hepatoma cells and fibroblasts were studied. It was confirmed by the characteristic fluorescence spectra that the protoporphyrin (PpIX) product could be produced in all three kinds of cells after cultured with ALA or He ALA. Laser scanning fluorescence microscopy showed that PpIX was diffusely distributed in the cytoplasm in neuroblastoma cells cultured with ALA or He ALA. PpIX accumulation dynamics in three different cells, with ALA or He ALA incubation time, PpIX accumulation in liver cancer cells and fibroblasts increased, while in neuroblastoma PpIX after 8h Saturated. In addition, under the same incubation conditions, the concentration of PpIX in neuroblastoma was significantly higher than that of hepatoma cells and fibroblasts. After ALA culture and light irradiation, almost 90% of neuroblastoma can be inactivated; while under the same conditions, it can only kill about 50% of liver cancer cells and fibroblasts. Revealed that neuroblastoma has a very high sensitivity to photodynamic therapy of ALA and is suitable for photodynamic therapy. Compared to ALA, He ALA produced similar kill rates to ALA in all three cells, but at a 10-fold lower drug concentration than ALA, He ALA showed very high photodynamic inactivation efficiency. Therefore, in endogenous photodynamic therapy, He ALA is a promising new drug.